pCEP4 Mammalian Expression Vector These vectors are designed for high-level, constitutive expression from either the CMV or RSV promoters. Both vectors contain the EBNA-1 Read more

pCEP4 Episomal mammalian vector with a CMV promoter and a hygromycin resistance marker, for high-level expression in primate and canine cells. Sequence Author : Thermo Fisher (Invitrogen)

pCEP4 is an episomal mammalian expression vector that uses the cytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site.

Plasmid pCEP4-M2L from Dr. James Thomson's lab contains the inserts MYC and LIN28 and is published in Science. 2009 Mar 26. This plasmid is available through Addgene.

pCEP4 vector is designed for high-level, constitutive expression from either the CMV or RSV promoters. Both vectors contain the EBNA-1 gene for episomal expression in primate and canine cell lines.

In preliminary experiments, we compared transient secreted placental alkaline phosphatase production in 8 cell lines from 3 different species using the pCIneo vs. pCEP4 vectors and found the utility of the pCEP4 vector to be limited to the human 293 EBNA cell line.

Comments for pCEP4 I 10186 base pairs CMV promoter: bases 1-588 Multiple cloning site: bases 619-676 SV40 polyadenylation signal: bases 685-926 OriP: bases 1344-3319 EBNA-1 gene (complementary strand): bases 3620-5545 Ampicillin resistance (bla) gene: bases 6171-7031 pUC origin: bases 7040-7815

Plasmid pCEP4-puroeGFP from Dr. Wesley Sundquist's lab contains the insert eGFP and is published in Curr Biol. 2022 Feb 25. pii: S0960-9822(22)00240-8. doi: 10.1016/j.cub.2022.02.018. This plasmid is available through Addgene.

Plasmid pCEP4 Smad2 from Dr. Bert Vogelstein's lab contains the insert Smad2 and is published in Nat Genet. 1996 Jul . 13(3):347-9. This plasmid is available through Addgene.

Provide pCEP4-tat vector/plasmid map, full length sequence, antibiotic resistance, size and other information