Pyridoxal-5′-phosphate (PLP), the active form of vitamin B 6, is an important and versatile coenzyme involved in a variety of enzymatic reactions, accounting for about 4% of all classified activities. However, the detailed catalytic reaction pathways for PLP-dependent enzymes remain to …

S型和R型ω-转氨酶结构中关键催化残基(Lys)相对辅酶PLP或结合底物的空间位置成镜像关系，这是引起酶立体选择性差异的关键因素。 图 1 ω-转氨酶结构及活性中心示意图 Figure 1 Structure and active center of ω-transaminase.

2011年1月28日 · Threonine synthase (TS), which is a pyridoxal 5′-phosphate (PLP)-dependent enzyme, catalyzes the elimination of the γ-phosphate group from O-phospho-l-homoserine (OPHS) and the subsequent addition of water at Cβ to form l-threonine. The catalytic course of TS is the most complex among the PLP enzymes, and it is an intriguing problem how the ...

Diaminopropionate (DAP) ammonia-lyase (DAPAL) is a prokaryotic PLP-dependent enzyme that catalyzes the degradation of d- and l -forms of DAP to pyruvate and ammonia. Here, we report the first crystal structure of DAPAL from Escherichia coli (Ec DAPAL) in tetragonal and monoclinic forms at 2.0 and 2.2 Å resolutions, respectively.

2017年10月16日 · Here we report the neutron crystal structure of a PLP-dependent enzyme, aspartate aminotransferase (AAT), a fold-type I PLP-dependent enzyme that reversibly converts l-aspartate and α ...

6 天之前 · The enzyme resting state is that of an “internal aldimine” or Schiff base, in which the PLP cofactor is covalently bound to the enzyme via a conserved Lys residue (P i = phosphate). The first ...

1992年2月24日 · The data indicate that Lys 190 is the primary PLP binding site. This Lys residue is distinct from other sites of covalent adduct formation; namely, the primary sites for nonenzymatic glycosylation (Lys 325) and acetylation by aspirin (Lys 199).

The present investigation identifies the molecular basis for the well-documented inhibition of the mitochondrial inner membrane citrate transport protein (CTP) function by the lysine-selective reagent pyridoxal 5'-phosphate. Kinetic analysis indicates …

2020年1月13日 · Pyridoxal-5′-phosphate (PLP), the active form of vitamin B 6, is an important and versatile coenzyme involved in a variety of enzymatic reactions, accounting for about 4% of all classified activities. However, the detailed catalytic reaction pathways for PLP-dependent enzymes remain to be explored.

1997年8月15日 · In this work we study the effects on the formation and hydrolysis of Schiff bases from PL, DPL and PLP with poly-L-lysine, of the hydrophobic medium derived on the presence of the macromolecule, in comparison with the behavior of the systems derived of the reaction between n-hexylamine and the same aldehydes [9]. 2.