PE-Cyanine®5 (PE-Cy5) is a red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy5. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy5. PE-Cy5 has an excitation peak at 496 nm and an emission peak at 667 nm.

PE-Cy5是流式细胞术中常用的颜色，荧光素在受到适当波长的光线照射时，会释放出波长比照射光长的光，这个现象叫做荧光。 激发光和释放… 切换模式

4) pe-cy5 ：pe-cy5是一种复合染料，属于藻红蛋白偶联物，在此类复合染料中，激发能量可从pe传递到cy5上，最大发射波长为667 nm。由于pe-cy5与fitc、pe在光谱上重叠范围较少，荧光干扰少，因此实验中常将pe-cy5与fitc、pe搭配使用。

2021年8月6日 · 2) pe-cy5：pe-cy5是一种复合染料，属于藻红蛋白偶联物，在此类复合染料中，激发能量可从pe传递到cy5上，最大发射波长为667 nm。由于pe-cy5与fitc、pe在光谱上重叠范围较少，荧光干扰少，因此实验中常将pe-cy5与fitc、pe搭配使用。

2017年8月28日 · PE-Cy5（TRI-COLOR，TC）又称TRI-COLOR或TC，是由PE和Cy5组合而成的Tandem荧光素；其标记的抗体适用于所有配备488nm氩离子激光器的流式细胞仪；PE-Cy5 的最大发射波长为670nm，在流式细胞仪上用FL3通道检测；PE-Cy5 标记的抗体可用于毫瓦级激光器的小流式细胞仪，也适用于 ...

2) PE-Cy5 ：PE-Cy5是一种复合染料，属于藻红蛋白偶联物，在此类复合染料中，激发能量可从PE传递到Cy5上，zui大发射波长为667 nm。由于PE-Cy5与FITC、PE在光谱上重叠范围较少，荧光干扰少，因此实验中常将PE-Cy5与FITC、PE搭配使用。

2021年8月28日 · PE-Cy5.5：PE-Cy5.5也是一种复合染料，能量从PE传递到Cy5.5上，最大发射波长为694 nm。 同PE-Cy5不一样，PE-Cy5.5与FITC、PE、APC间荧光干扰小，可搭配使用，且其荧光强度要优于PE-Cy5。 PE-Cy7：复合染料，最大发射波长为785 nm。 PE-Cy7与FITC无光谱重叠，与APC重叠也较少，因此其可与FITC、PE、APC搭配使用。 在实验中需要注意，PE-Cy7的光淬灭性很强，需要绝对避光环境。 PerCP-Cy5.5：复合染料，最大发射波长为695 nm。

PE-Cy5 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by the 488 nm light of an Argon ion laser and serves as an energy donor, coupled to the cyanine dye Cy5, which acts as an energy acceptor and fluoresces at 670 nm.

PE-Cy5串联染料是美国AAT Bioquest生产的 荧光染料。 PE-Cy5是流式细胞术中常用的颜色。 其主要吸收峰位于565nm，发射峰位于666nm。 对于这种串联颜色，建议使用682/33 nm和695/40 nm的滤光片组。 AAT Bioquest还提供独特的预活化PE-Cy5，以促进PE-Cy5串联结合抗体和其他蛋白质，如链霉抗生物素蛋白和其他二级试剂。 我们的预活化PE-Cy5串联已准备好结合，产生比常规繁琐的基于SMCC的缀合化学高得多的产率。 此外，我们的预活化PE-Cy5串联通过其蛋 …

2025年2月12日 · PE - Cy5 复合染料：PE - Cy5 是藻红蛋白与其他物质偶联形成的复合染料，内部有独特的能量传递机制，激发能量能从 PE 顺利 “转移” 到 Cy5 上，它的最大发射波长达到 667nm。在多色荧光搭配实验里，它和 FITC、PE 是 “好搭档”，光谱重叠少，彼此 “工作” 时干扰 ...

2025年3月11日 · PE-Cy5 is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at 666 nm. The filter sets of 682/33 nm and 695/40 nm are recommended for this tandem color.

PE-Cy5 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by the 488 nm light of an Argon ion laser and serves as an energy donor, coupled to the cyanine dye Cy5, which acts as an energy acceptor and fluoresces at 670 nm.

PE-Cy5 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by the 488 nm light of an Argon ion laser and serves as an energy donor, coupled to the cyanine dye Cy5, which acts as an energy acceptor and fluoresces at 670 nm.

Streptavidin-PE/Cyanine5 is useful for detecting biotinylated antibodies. The excitation of PE/Cyanine5 by 488 nm laser light induces a light emission maximum of 670 nm. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Streptavidin is conjugated with PE/Cyanine5 under optimal conditions.

PE (phycoerythrin)-Cy5 is a fluorescent compound with an excitation peak at 565 nm and an emission peak at 666 nm. Other spectra of interest include: PE (phycoerythrin)-iFluor 647, PE (phycoerythrin)-Cy5.5, and PE-iFluor™ 660.

2024年10月24日 · PE CY5，也称为PE-Cyanine5，是一种在流式细胞术和其他生物学实验中广泛使用的串联染料。 它结合了R-PE（一种从红藻中纯化的藻胆蛋白）和花青染料Cy5，形成了一种具有**荧光性能和稳定性的荧光染料。

Our preactivated PE-Cy5 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PE-Cy5 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the ...

PE-Cyanine®5 (PE-Cy5) is a red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy5. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy5. PE-Cy5 has an excitation peak at 496 nm and an emission peak at 667 nm.

PE-Cyanine®5.5 (PE-Cy5.5) is a red-emitting tandem fluorophore that combines phycoerythrin (PE) and Cy5.5. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Cy5.5. PE-Cy5.5 has an excitation peak at 496 nm and an emission peak at 695 nm.