Find high-capacity nickel columns and resins for rapid purification of recombinant histidine-tagged proteins. Optimal ligand density for high purity.

Nickel is the most widely available metal ion for purifying His-tagged proteins. Nickel generally provides good binding efficiency to His-tagged proteins but also tends to bind nonspecifically to endogenous proteins that contain histidine clusters.

NEBExpress Nickel Spin Columns are pre-packed with agarose-based microparticles ranging in size from 10–100 µm for the small-scale isolation and purification of His-tagged fusion proteins.

Ni-NTA Spin Columns (His-protein purification spin columns) in the Ni-NTA Spin Kit and available separately provide Ni-NTA silica in a convenient microspin format for easy preparation of multiple samples in parallel.

2021年10月12日 · Nickel column is a purification column material frequently used in protein purification and plays an important role in nickel column affinity chromatography.

Thermo Scientific HisPur Ni-NTA Spin Columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins. Features of HisPur Ni-NTA Resin: • High capacity—bind up to 60 mg of 6xHis-tagged protein per milliliter of resin

2019年6月17日 · Learn how to decide between nickel- and cobalt-based IMAC resins for your his-tagged purification.

Add an appropriate amount of Ni-NTA Resin to a suitable column (suitable to hold 7 columns volumes (CV)). Allow the storage buffer to flow through the column or centrifuge at 800xg for 1 minute. Discard the flow-through.

High Affinity Ni-NTA Resin (Cat. No. L00250, 25 ml as 50 ml of 50% slurry) is an agarose resin (4% cross-linked) covalently coupled to a tridentate chelating agent (NTA) that binds Ni2+ ions by four coordination sites for high-affinity purification of His-tagged recombinant proteins without leacking of Ni2+.

Capturem his-tagged columns are compatible with a wide range of additives while quickly purifying concentrated recombinant proteins. Learn about the buffers compatible with Capturem his-tagged purifications.