2020年6月8日 · These studies suggest that increased neutrophil count can contribute to CRS and lung damage in patients with ARDS. Additionally, elastase secreted by these cells is one of the key proteolytic enzymes shown to activate the spike (S) protein of coronaviruses and shift the viral entry to a low pH-independent route .

2004年3月15日 · We determined the effect of r-SPC with and without elastase inhibitor (EI) on neutrophil apoptosis. Heparinised venous blood samples were obtained from 10 healthy adult volunteers, and PMNL were isolated by density gradient centrifugation and suspended in minimum essential medium supplemented with 10% autologous plasma.

2014年7月9日 · Neutrophil elastase associated with NETs cleaves tissue factor pathway inhibitor (TFPI) [63, 64], a primary inhibitor of TF that is synthesized mainly by endothelial cells and platelets and is presented in the blood as well as on cell surfaces. The increased procoagulant activity induced by the degradation of TFPI is crucial for preventing the ...

2021年10月12日 · Miyazaki Y, Inoue T, Kyi M, Sawada M, et al. Effects of a neutrophil elastase inhibitor (ONO-5046) on acute pulmonary injury induced by tumor necrosis factor alpha (TNFalpha) and activated neutrophils in isolated perfused rabbit lungs. Am J Respir Crit Care Med. 1998;157(1):89–94. Article CAS Google Scholar

2020年6月29日 · Background Platelets have been demonstrated to be potent activators of neutrophil extracellular trap (NET) formation during sepsis. However, the mediators and molecular pathways involved in human platelet-mediated NET generation remain poorly defined. Circulating plasma exosomes mostly originating from platelets may induce vascular apoptosis and …

2024年10月31日 · Inflammatory cells release neutrophil elastase and matrix metalloproteinase (MMPs), disrupting the lung's proteinase/anti-proteinase balance and causing the degradation of lung elastin and connective tissue . ECM remodeling and altered collagen deposition further contribute to lung function decline.

2016年2月17日 · Ulinastatin, a urinary trypsin inhibitor (UTI) that inhibits various inflammatory proteases, including chymotrypsin, trypsin, and neutrophil elastase, has been widely used in China, Japan, and Korea for the treatment of patients with inflammatory disorders, postoperative organs protection, shock, and pancreatitis [17–19].

2016年1月12日 · Background Leukocyte-mediated pulmonary inflammation is a key pathophysiological mechanism involved in acute respiratory distress syndrome (ARDS). Massive sequestration of leukocytes in the pulmonary microvasculature is a major triggering event of the syndrome. We therefore investigated the potential role of leukocyte stiffness and …

2016年3月23日 · For instance, neutrophil elastase reduces expression of co-stimulatory molecules by dendritic cells, limiting maturation and induction of a proper Th1 response . In addition, T cells in the inflammatory microenvironment may be affected by neutrophil elastase by cleavage of their IL-2 and IL-6 receptors (Fig. 1a) . Another mechanism of immune ...

2022年3月22日 · Levels of MMP-2, MMP-9, and neutrophil elastase measured in plasma were also not affected by CMT-3 in a cecal ligation and puncture-induced ARDS model . The authors concluded that this might be due to the use of ketamine, which has been shown to weaken the effects of cecal ligation and puncture in rats via the inhibition of NF-κB.