Jul 11, 2022 · Nap1 is a conserved protein in eukaryotes involved in diverse physiological processes, such as nucleosome assembly, histone shuttling between the nucleus and cytoplasm, transcriptional regulation, and the cell cycle. Here, we identified Nap1 and characterized its roles in fungal development and virulence in M. oryzae. MoNap1 is involved in ...

Nov 16, 2020 · Our results provide evidence of multiple interaction modes of a NAP1-family histone chaperone, thereby shedding light on how histone chaperones effectively shield H2A-H2B from nonspecific interaction with DNA.

The nucleosome assembly protein Nap1 is used extensively in the chromatin field to reconstitute nucleosomal templates for structural and functional studies. Beyond its role in facilitating experimental investigation of nucleosomes, the highly conserved Nap1 is one of the best-studied members of the …

Jul 11, 2022 · Nap1 is a conserved protein in eukaryotes involved in diverse physiological processes, such as nucleosome assembly, histone shuttling between the nucleus and cytoplasm, transcriptional regulation, and the cell cycle. Here, we identified Nap1 and characterized its roles in fungal development and virulence in M. oryzae.

Mar 26, 2010 · Furthermore, our studies reveal the mechanism of action of the histone chaperone nucleosome assembly protein 1 (Nap1). We present evidence for a paradigm in which nucleosome assembly requires the elimination of competing, nonnucleosomal histone-DNA interactions by Nap1.

To demonstrate the versatility of our approach, we test the effect of DNA sequence and H3K56 acetylation on nucleosome thermodynamics. Furthermore, our studies reveal the mechanism of action of the histone chaperone Nucleosome Assembly Protein 1 (Nap1).

Jun 8, 2020 · Here, we show that NRP proteins cause a decrease of H2A.Z-containing nucleosomes in Arabidopsis under standard growing conditions. nrp1-1 nrp2-2 double mutants show an over-accumulation of H2A.Z...

Jan 13, 2025 · NAP1 impacts gene expression by shaping chromatin landscapes, dictating transcriptional machinery access to genomic regions. This modulation enables dynamic gene expression regulation in response to developmental cues and environmental changes.

Sep 18, 2015 · Nucleosome assembly protein 1 (NAP1) represents a highly conserved histone chaperone from yeast to human, which functions in assisting the traffic of histones, directly impacting nucleosome dynamics [9], [10], [11], [12], [13].

Nap1 is essential for maintaining proper chromatin composition and modulating the exchange of H2A-H2B in vivo. these results define the distinct roles for histone chaperones Chz1 and Nap1 to regulate Htz1 incorporation into chromatin.