The growth of mycelium begins with inoculation, where a selected strain of mushroom spawn is introduced to a substrate. Under optimal conditions of moisture, temperature, and oxygen, the mycelium spreads and colonizes the substrate, breaking down complex compounds into simpler nutrients that will later support mushroom formation.

The Steps to Growing Mushrooms from Mycelium Obtain a Spore or Culture (mycelium) syringe Expand your Syringe to create unlimited supply (non-essential) Innoculate your sterilized spawn with liquid culture Spawn into a bulk substrate Wait Induce fruiting conditions

Mycelial expansion is the next stage in mushroom growth When compatible hyphae fuse, they form a network called “mycelium”. The mycelia are essentially the mushroom roots that have a major role in the mushroom life cycle as well as the ecosystem. They are formed 7–14 days after inoculation.