mRuby3 is a basic (constitutively fluorescent) red fluorescent protein published in 2016, derived from Entacmaea quadricolor. It has low acid sensitivity.

2016年2月16日 · We show that mRuby3 is the brightest and most photostable monomeric RFP to date, and additionally offers high FRET efficiency in GFP-RFP fusions. Further, we find that mClover3 is a...

Monomeric red fluorescent protein derived from mRuby2, with improved brightness and photostability. Explore Over 2.7k Plasmids: Fluorescent Protein Genes & Plasmids | More Plasmid Sets. BsrGI is typically used at 37°C, but is even more active at 60°C. Sticky ends from different BseRI sites may not be compatible.

2020年2月1日 · Here we established the fluorescent protein mRuby3 as tag for efficient in vivo protein localization studies by expressing a codon-optimized gene in P. tricornutum. mRuby3 was directed to seven different subcellular localizations by means of full-length marker protein or N-/C-terminal targeting signal fusions; its emission was detected ...

2009年2月5日 · With excitation and emission maxima at 558 nm and 605 nm, respectively, and a large Stokes shift of 47 nm, mRuby appears particularly useful for imaging applications. The protein shows an exceptional resistance to denaturation at pH extremes.

2024年2月14日 · We are announcing the first stable release of mruby 3.3 series - mruby 3.3.0. Describes the new features and changes in mruby 3.3. The main changes in mruby 3.3 are also described in doc/mruby3.3.md. “ NOTE:” are changes to be aware of. mruby can be built using Docker now. Try docker-compose build for example. (#5961)

Our results show the potential of mRuby3 for its application in studying protein targeting and localization in P. tricornutum, particularly underlining its compatibility with GFP and the plastid autofluorescence in signal detection.