MitoSOXTM Green (MSG) reagent and MitoSOXTM Red (MSR) reagent are live-cell permeant which rapidly and selectively target the mitochondria. Once in the mitochondria, the MSG reagent or MSR reagent are oxidized by superoxide and exhibit bright green or …

MitoSOX superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells. Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence.

In this ROS Protocols article, we described the flow cytometric protocol involving the use of various concentrations of MitoSOX (1, 2.5, 5 μM) for detecting mitochondrial ROS in control and mitochondrial DNA-deficient (MD) melanoma B16-F10 cells.

Hypoxia is known to stimulate mitochondrial reactive oxygen species (mROS) in cells. Here, we present a detailed protocol to detect mROS using MitoSOX staining in live cells under normoxia and hypoxia. Flow cytometry allows sensitive and reliable quantification of …

Dissolve one vial of MitoSOX (50 micrograms) into 13 microliters of DMSO. This makes a 5mM MitoSOX solution. Add 5mL of HBSS to entire tube. 0.25mL per 24-well plate. 0.5 per 12-well plate. Incubate at 37 degrees Celsius protected from light …

We describe use dyhydroethidium (DHE) and MitoSOX (a derivative of dihydroethidium bound to a lipophilic cation) to detect mitochondrial superoxide in yeast and mammalian cells, respectively. We also describe use of genetically encoded fluorescent biosensors for quantitative analysis of mitochondrial NADPH levels (iNap) in mammalian cells and ...

Hypoxia is known to stimulate mitochondrial reactive oxygen species (mROS) in cells. Here, we present a detailed protocol to detect mROS using MitoSOX staining in live cells under normoxia and hypoxia. Flow cytometry allows sensitive and reliable quantification of …