A common source is E.coli cells carrying a cloned nuc gene encoding Staphylococcus aureus extracellular nuclease (micrococcal nuclease).

碧云天生产的微球菌核酸酶(Micrococcal Nuclease, MNase)，也被称为Micrococcal Endonuclease或S7 Nuclease，是一种来源于金黄色葡萄球菌(Staphylococcus aureus)的核酸内切酶，CAS号为9013-53-0，分子量约18.6k

Micrococcal nuclease is derived from Staphylococcus aureus and is a relatively non-specific endo-exonuclease. It is purified from a recombinant E. coli strain that digests double-stranded, single-stranded, circular and linear nucleic acids.

Also known as Staphylococcal Nuclease; Need help finding the right exonuclease for your experiments? Try Exo Selector ; Micrococcal Nuclease is ideal for: Preparation of double-stranded DNA fragments with 5'-OH and 3'-phosphate ; Studies of chromatin structure; Degradation of nucleic acids in crude cell-free extracts; Preparation of rabbit ...

微球菌核酸酶 （Micrococcal nuclease；MNase）是一种可以降解核小体连接区DNA的核酸酶，MNase 消化染色质可以释放出一个个独立的核小体。因此用MNase 酶解染色质是研究核小体在基因组上定位的重要工具。

Thermo Scientific 微球菌核酸酶 (MNase) 适用于从细胞裂解物中去除核酸，释放染色质结合蛋白并剪切染色质以便用于染色质免疫沉淀 (ChIP) 实验。 该微球菌核酸酶是金黄色葡萄球菌衍生酶的一种稳定液体形式。 微球菌核酸酶 (MNase) 表现出针对 DNA 和 RNA 的外切和内切-5'-磷酸二酯酶活性。 该酶可消化各种双链、单链、环状和线性核酸。 最高活性面向单链核酸底物，并优先针对AT-或AU-富集区域。 酶活性发生于 pH 值 7 至 10 条件下，且在酶切 RNA 和 DNA 底物方面完全 …

微球菌核酸酶(Micrococcal Nuclease) 是只降解核小体连接区DNA的核酸酶，由于核小体处的DNA被组蛋白1保护而不被降解。

Micrococcal nuclease (MNase) is an endonuclease–exonuclease that digests DNA without bound proteins or secondary structures. The end product of MNase digestion is a collection of nucleosomes; hence, MNase-seq results in DNA sequences that are wrapped around core histones [ 169–171 ].

Micrococcal Nuclease (S7 Nuclease) is a relatively nonspecific endo-exonuclease that digests single-stranded and double-stranded nucleic acids, but is more active on single-stranded substrates. Cleavage of DNA or RNA occurs preferentially at AT or AU-rich regions, yielding mononucleotides and oligonucleotides with terminal 3'-phosphates.

本酶是一种核酸内切酶，对单链核酸的作用较好，特别是在富含AT或AU的区域。 也能分解双链DNA或RNA。 本酶消化DNA或RNA的5’磷酸键产生3’磷酸的单核苷酸和寡核苷酸。 本酶的催化作用需要Ca2+的存在，用EDTA或EGTA可使其完全失活。 -20℃。 Staphylococcus aureus菌体的培养液。 以热变性小牛胸腺DNA作底物，在37℃ 、pH8.0的条件下，30分钟生成1 OD260的酸可溶性物质所需要的酶量定义为一个活性单位 (unit)。 1. 细胞粗抽提液中核酸的水解。 2. 为制备 …