Here, we describe a detailed methodology on how to develop MCAO stroke in rodents using intra-arterial insertion of filament to occlude the middle cerebral artery. This approach allows for the …

The intraluminal monofilament model of middle cerebral artery occlusion (MCAO) involves the insertion of a surgical filament into the external carotid artery and threading it forward into the …

Here we describe (and present in the video) how to successfully perform the MCAo model (with reperfusion) in mice to generate reliable and reproducible data. Stroke is the fifth leading …

2023年6月23日 · Ischemic stroke in rodents is usually induced by intraluminal middle cerebral artery occlusion (MCAO) via the common carotid artery plugging filament invented by Koizumi …

Creative Biolabs provides both permanent MCAO model which produces massive damage (e.g., cortex stratum, thalamic nucleus) and transient MCAO models where the artery is occluded for …

2011年2月13日 · To evaluate the extent of cerebral infarction, we stain brain slices with 2,3,5-triphenyltetrazolium chloride (TTC) to identify ischemic brain tissue. In this video, we …

2011年5月8日 · The intraluminal middle cerebral artery occlusion (MCAO) model was developed by Koizumi in 1986 to simulate this impactful human pathology in the rat. A modification of the …

2022年12月9日 · Intraluminal monofilament model of middle cerebral artery occlusion (MCAO) is widely adopted for ischemic stroke; and Sprague–Dawley (SD) rats are commonly used …

2019年7月24日 · Rodent transient middle cerebral artery occlusion (MCAO) is one of the widely used focal ischemia models in the world. This model mimics human cerebral ischemic injury …

2023年6月29日 · The middle cerebral artery occlusion (MCAO) model is currently the most widely used animal model of ischemic stroke. This review discusses various methods for constructing …