Multilocus sequence typing (MLST) is an unambiguous procedure for characterising isolates of bacterial species using the sequences of internal fragments of (usually) seven house-keeping genes. Approximately 450-500 bp internal fragments of each gene are used, as these can be accurately sequenced on both strands using an automated DNA sequencer.

Multilocus sequence typing (MLST) is a technique in molecular biology for the typing of multiple loci, using DNA sequences of internal fragments of multiple housekeeping genes to characterize isolates of microbial species.

We welcome submissions to the databases we host. Submissions may consist of allele sequences, MLST profiles, or isolate records with or without genome assemblies.

Marine Life Science & Technology (MLST) provides a prime platform for new discoveries and theories in marine and aquatic life sciences and technologies. Encourages multidisciplinary approaches across a broad range of life sciences and technologies.

Multilocus sequence typing (MLST) was first proposed in 1998 as a typing approach that enables the unambiguous characterization of bacterial isolates in a standardized, reproducible, and portable manner using the human pathogen Neisseria ...

2023年10月6日 · Multilocus sequence typing (MLST)-Introduction, Principle, Test Requirements, Procedure, Result-Interpretation, Application, and Keynotes

Multi-locus sequence typing (MLST) is a high-resolution genetic typing approach to identify species and strains of pathogens impacting human health, agriculture (animals and plants), and biosafety.

MLST (multi-locus sequence typing) is a classic technique for genotyping bacteria, widely applied for pathogen outbreak surveillance. Traditionally, MLST is based on identifying sequence types from a small number of housekeeping genes.

MLST was first developed for Neisseria meningitidis in 1998 to overcome the poor reproducibility between laboratories of older molecular typing schemes . The principle behind the MLST scheme is to identify internal nucleotide sequences of approximately 400 to 500 bp in multiple housekeeping genes.

Multilocus sequence typing (MLST) is a widely used system for typing microorganisms by sequence analysis of housekeeping genes. The main advantage of MLST in comparison to other typing techniques is the unambiguity and transferability of sequence data.