The RayBiotech m7G dot blot assay provides a simple and rapid method of quantifying the global m6A levels in RNA/DNA, urine, serum, plasma, or other nucleic samples collected from all species.

相比于蛋白质印迹技术， 斑点印迹分析法 (Dot Blotting)通过简化实验流程，可以 完成高通量样本蛋白质表达水平的检测，但是实验结果依然只能以“半定量”形式体现。 在斑点印迹中，要检测到的生物分子不是首先通过电泳分离出来的，而是将含有被检测分子的混合物直接点在膜上，烘烤固定。 然后用 核苷酸探针 或 抗体杂交 后检测。 这种方法耗时短，可做半定量分析。 一张膜上可 …

2024年8月19日 · m7G 甲基化作为一种普遍存在且进化保守的 RNA 修饰，在早前的研究中被认为主要发生在真核细胞 mRNA 的 5′ 端鸟苷 N7 位点，介导转录延长、mRNA 剪接、出核和翻译等过程。 m7G 修饰通过影响各种 RNA 分子的代谢，包括 mRNA、tRNA、microRNA 和核糖体 RNA。 越来越多的证据表明，m7G 在人类疾病的发展中发挥着关键作用。 目前 m7G 中标的项目越 …

dot blot is a powerful tool for analyzing m7G. This kit is highly sensitive and specific, requires minimal sample preparation, no specialty equipment, and can be completed within 3-4 hours, m.

Here, we introduce a combined m1A, m7G, m6A, and m5C dot blot kit, which offers a reliable, simple, and rapid method of quantifying the 4 most common global RNA modification levels in RNA, urine, serum, plasma, or other nucleic samples collected from humans, rats, and mice.

2023年3月14日 · Here, we review recent findings regarding the distribution, abundance, biogenesis, modifiers, and functions of m7G modifications. We also provide an up-to-date summary of m7G detection and profile mapping techniques, databases for validated and predicted m7G RNA sites, and web servers for m7G methylation prediction.

2025年2月3日 · Aldevron assessed the samples via semi-quantitative m7G dot blot assay for comparison, and intactness/integrity measurements were made via capillary electrophoresis. As shown in Table 2, all four...

Dot blots indicated that m 7 G levels were elevated for eIF4E-Flag cells in both compartments relative to vector controls or S53A-Flag cells (SI Appendix, Fig. S2 A and B).

The RayBio® combined 4-RNA modifications dot blot Kit is an in vitro semi-quantitative assay for detecting m1A, m7G, m6A and m5C. The RNA-containing sample is directly spotted onto a nitrocellulose (NC) or nylon membrane followed by incubation with their individual primary and secondary antibodies.

Simply and rapidly detect m6A, m5C, m7G or m1A RayBiotech's dot blot assays provide a simple and rapid method of quantifying the levels of global methylations in RNA/DNA, urine, serum, plasma, or other nucleic samples collected from humans, rats, and mice.