Feb 21, 2021 · Recent studies have shown that m6A RNA modification plays essential role in both physiological and pathological conditions, especially in the initiation and progression of different types of...

Dec 12, 2024 · N6-methyladenosine (m6A) is an mRNA modification influencing gene expression. Advanced methodologies for mapping m6A enhance understanding of its dynamic roles and interactions.

Sep 13, 2019 · RNA methylation to form N 6 -methyladenosine (m 6 A) in mRNA accounts for the most abundant mRNA internal modification and has emerged as a widespread regulatory mechanism that controls gene...

Feb 1, 2021 · We highlight advances in our understanding of the regulation of m 6 A deposition on mRNA and its context-dependent effects on mRNA decay and translation, the role of m 6 A methylation of non-coding chromosomal-associated RNA species in regulating transcription, and the activities of the RNA demethylase FTO on diverse substrates.

We highlight advances in our understanding of the regulation of m 6 A deposition on mRNA and its context‐dependent effects on mRNA decay and translation, the role of m 6 A methylation of non‐coding chromosomal‐associated RNA species in regulating transcription, and the activities of the RNA demethylase FTO on diverse substrates.

Mar 12, 2025 · N6-methyladenosine (m6A) is a prevalent and conserved RNA modification in eukaryotes. While its roles in the 3’ untranslated regions (3’ UTR) are well-studied, its role in the 5' UTR and its relationship with histone modifications remain underexplored. We demonstrate that m6A methylation in the 5’ UTR of mRNA triggers a downstream shift in H3K4me3 modification.

Feb 21, 2021 · N 6 -methyladenosine (m6A) is the most prevalent, abundant and conserved internal cotranscriptional modification in eukaryotic RNAs, especially within higher eukaryotic cells. m6A modification is modified by the m6A methyltransferases, or writers, such as METTL3/14/16, RBM15/15B, ZC3H3, VIR …

Jan 29, 2022 · Here, we review the general role of m6A, highlighting recent advances related to the three major types enzymes that determine the level of m6A modification (i.e., writers, erasers, and readers) and the regulatory mechanism by which m6A influences multiple stages of …

Of note, m6A mRNA granules have been observed within 30 minutes of heat shock. Thus, some m6A mRNAs may still be preferentially enriched in these granules for storage rather than being directly degraded. Overall, it would be interesting to understand the authors' position relative to previous studies of m6A during heat stress.

3 days ago · Purpose N6-methyladenosine (m6A) is the most prevalent base epigenetic modification within eukaryotic mRNAs. It participates in post-transcriptional regulation, including maternal RNA maintenance and decay in mouse oocytes and during maternal-to-zygotic transition. The landscape in other mammalian species remains largely unexplored. The …

Sep 17, 2020 · Through the development of m6A RNA sequencing, the relevant molecular mechanism of m6A modification has gradually been revealed. It has been found that the effect of m6A modification on RNA metabolism involves processing, nuclear …

Sep 9, 2024 · Brown adipose tissue (BAT) regulates systemic metabolism by releasing signaling lipids. N 6 -methyladenosine (m 6 A) is the most prevalent and abundant post-transcriptional mRNA modification and has been reported to regulate BAT adipogenesis and energy expenditure.

m 6 A is a reversible modification in mRNA (reviewed in ), meaning that distinct sets of proteins introduce (writers), recognize (readers) and remove (erasers) this mark, allowing it to exert reversible regulation of mRNA metabolism.

Mar 12, 2025 · Results: We demonstrate that m 6 A methylation in the 5' UTR of mRNA triggers a downstream shift in H3K4me3 modification. This regulatory mechanism is conserved in Arabidopsis, rice, and chrysanthemum. The observed shift in H3K4me3 is genetically controlled by m 6 A modifiers and influences gene expression.

As the most prevalent internal modification in mRNA, N 6-methyladenosine (m6A) plays broad biological functions via fine-tuning gene expression at the post-transcription level. Such modifications are deposited by methyltransferases (i.e., m6A ...

Mar 25, 2022 · The mRNA of Nfkbia, the NF-κB inhibitor, is the direct target of m 6 A modification. Thus, depletion of m 6 A modification led to enhanced stability of Nfkbia mRNA and accumulation of NFKBIA protein, which down-regulated the NF-κB–mediated antiapoptotic pathway and promoted colonic cell death.

Feb 15, 2024 · Here we dissect the determinants governing RNA methylation via interspecies and intraspecies hybrids in yeast and mammalian systems, coupled with massively parallel reporter assays and m6A-QTL reanalysis.

Mar 10, 2025 · Here, we demonstrated that a critical m6A reader, insulin like growth Factor 2 mRNA binding protein 3 (IGF2BP3) directly recognized m6A modified mRNAs of MCM10 gene and stabilized MCM5 mRNAs to ...

Feb 2, 2023 · Here, we review RNA m6A modification in the light of the recent appreciation of nuclear roles for m6A in regulating chromatin states and gene expression, as well as the recent discoveries of the evolutionarily conserved methyltransferases, which catalyze methylation of adenosine on diverse sets of RNAs.

3 days ago · The m6A level of YPEL3 mRNA was analyzed using the Magna MeRIP m6A Kit (Millipore, Billerica, MA, USA). Briefly, RNA was extracted from THLE-2 and AML12 cells and quantified with the Nanodrop spectrophotometer. Magnetic beads/anti-m6A antibody complex was prepared by adding 10 µg of the anti-m6A antibody into magnetic beads and incubating for ...

Jun 16, 2022 · In this review, Murakami et al. discuss the recently recognized concept of epitranscriptomic regulation with a focus on N6-methyladenosine (m6A), the most abundant internal mRNA modification.

N6-methyladenosine (m6A) is the most frequent chemical modification in eukaryotic mRNA and is known to participate in a variety of physiological processes, including cancer progression and viral infection. The reversible and dynamic m6A modification ...

Though N6 -methyladenosine (m 6 A) modification has been well known to affect the fate of messenger RNA (mRNA), whether it plays a role in regulating plant immunity remained unclear. Our study demonstrates that m 6 A modification of mRNA is …

Jan 20, 2021 · We highlight advances in our understanding of the regulation of m 6 A deposition on mRNA and its context‐dependent effects on mRNA decay and translation, the role of m 6 A methylation of non‐coding chromosomal‐associated RNA species in regulating transcription, and the activities of the RNA demethylase FTO on diverse substrates.

Geula et al. now show that N6-methyladenosine (m6A), a messenger RNA (mRNA) modification present on transcripts of pluripotency factors, drives this transition. Methylation destabilized mRNA transcripts and limited their translation efficiency, which promoted the timely decay of naïve pluripotency.

Mar 11, 2025 · Background The screening and monitoring of gastric cancer is still a clinical challenge. Both N6-methyladenosine (m6A) and lncRNAs have been evidenced as critical regulators of gastric cancer, but their interaction and potential in modulating tumor progression remain unclear. This study aimed to evaluate the function of lncRNA LINC00968 in gastric …

Sep 21, 2023 · As the most common internal post-transcriptional RNA modification in eukaryotic cells, N6-methyladenosine (m 6 A) performs a dynamic and reversible role in a variety of biological processes mediated by methyltransferases (writers), demethylases (erasers), and m 6 A binding proteins (readers).