Liquid chromatography (LC) separates molecules in a liquid mobile phase using a solid stationary phase. Liquid chromatography can be used for analytical or preparative applications. Here, we restrict our discussion to column liquid chromatography techniques and considerations.

2016年2月1日 · overcoming some of the common problems that can occur with columns and fittings in everyday use. The guide covers the main columns used in LC, with particular emphasis on reversed-phase high performance liquid chromatography. How to use this guide: • Sections are color-coded for your easy reference.

2023年8月29日 · Liquid-solid column chromatography, the most popular chromatography technique and the one discussed here, features a liquid mobile phase which slowly filters down through the solid stationary phase, bringing the separated components with it.

Explore our expansive range of prepacked and empty liquid chromatography columns for lab- and process-scale purification workflows. Columns are available in a wide selection of pressure ratings and sizes, and are compatible with common chromatography systems and …

2024年1月5日 · Choosing the right chromatographic column for High-Performance Liquid Chromatography (HPLC) is crucial for achieving successful separation of your analytes. Here’s a guide to help you make the best choice:

Columns can function in ambient air but are generally thermostatted and housed within a temperature-controlled column compartment. Proper column temperature control is essential to conserving retention time precision, selectivity, and separation efficiency.

Resolve your toughest separation challenges with Waters analytical and preparative HPLC columns, which come in 5,800+ column formats, 175+ column configurations, and 21 unique column phases — for excellent performance and column-to-column reproducibility.

2023年5月13日 · In this blog post, we will be discussing the different types of columns used in High-Performance Liquid Chromatography (HPLC). We will explain what they are, how they work, and what their main benefits and drawbacks are.

Acclaim™ HPLC columns are based on high-purity, porous silica particles (2.2, 3.0, 5.0µm), with advanced and innovative column bonding technologies. This provides complementary selectivity, high column efficiencies, and symmetrical peaks.

2024年5月31日 · LC columns are the backbone of liquid chromatography, influencing the separation process's efficiency, resolution, and overall success. Understanding the different types of columns, their applications, and proper maintenance techniques is vital for anyone working in analytical chemistry.