One of these methods is injecting directly into cerebral lateral ventricles (Intracerebroventricular) which results in delivery of materials into the CNS through the cerebrospinal fluid 3,4. The second method is a temporal vein injection (intravenous) that can introduce different therapeutics into the circulatory system, leading to systemic ...

If a mouse appears to be in pain or distress, we recommend providing the mouse with a 5 mg/kg subcutaneous injection of Carprofen once every 24 hr for up to 5 days in order to relieve the pain. If there appears to be an infection, generously apply antibiotic ointment to the area daily and consult an Attending Veterinarian to ensure that the ...

Jan 12, 2024 · Here, methods for making free-hand ICV injections in adult mice are described. By using visual and tactile landmarks on the heads of mice, injections into the lateral ventricles can be made rapidly and reliably. The injections are made with a glass syringe held in the experimenter's hand and placed at approximate distances from the landmarks.

Passini MA, et al. Intraventricular brain injection of adeno-associated virus type 1 (AAV1) in neonatal mice results in complementary patterns of neuronal transduction to AAV2 and total long-term correction of storage lesions in the brains of beta-glucuronidase-deficient mice.

Mar 17, 2023 · Experimentally targeting mouse choroid plexus (ChP) provides a valuable approach for investigating mechanisms of ChP-cerebrospinal fluid (CSF) biology. Here, we provide a protocol to deliver adeno-associated viral vectors (AAVs) by in utero intracerebroventricular (ICV) injection to

Oct 3, 2011 · The purpose of this video is to demonstrate two different injection paradigms to deliver therapeutic materials into neonatal mice soon after birth. One of these methods is injecting directly into cerebral lateral ventricles (Intracerebroventricular) which results in delivery of materials into the CNS through the cerebrospinal fluid.

May 1, 2017 · Application of Aβ in the lateral ventricle of mice is associated with cognitive impairment of declarative memory in the mHBT. There is no interference caused by altered behaviour. Therefore, it represents a valid model for acute Aβ-mediated neurotoxic effects.

Intracerebroventricular (ICV) administration of cells in mouse models is an efficient tool for exploring the therapeutic efficacy of cells injected directly into the central nervous system (CNS) and thus bypassing the blood-brain barrier.

The BBB can be bypassed by administering directly into the brain. Here we describe a method to perform single and repeated intracerebroventricular injections into the lateral ventricle of the mouse brain.

Apr 1, 2017 · To achieve widespread drug delivery in the CNS, MOs can be administered through a single bolus ICV injection into the mouse cerebral ventricular system. The ICV injection ensures a direct delivery into cerebrospinal fluid (CSF) via …