LA Taq DNA polymerase—for long-range PCR - Takara Bio
LA Taq DNA polymerase is a mixture of Taq and proofreading polymerases that enables PCR amplification of very long DNA templates (up to 48 kb). It is supplied with optimized buffers and dNTPs, and can be used for genomic DNA or GC-rich templates.
Long and accurate polymerase chain reaction - Nature Methods
Long and accurate polymerase chain reaction (LA PCR) refers to the production of amplified product longer than ∼ 3 kilobases (kb) with high fidelity. Long PCR mixtures typically yield...
Long and Accurate Polymerase Chain Reaction (LA PCR)
2019年3月1日 · Long and accurate PCR (LA PCR) addresses the issue in part by using a mixture of two different thermostable DNA polymerases to catalyze the amplification reaction.
Principles and applications of polymerase chain reaction in …
PCR is an excellent technique for the rapid detection of pathogens, including those difficult to culture. Along with conventional PCR techniques, Real-Time PCR has emerged as a technological innovation and is playing an ever-increasing role in …
Long and Accurate Polymerase Chain Reaction (LA PCR) - CSH …
The standard polymerase chain reaction (PCR) is easily capable of amplifying segments of DNA smaller than ∼3 kb in length—sufficient for most purposes, but not enough to amplify an entire mammalian gene, nor even a cDNA of average dimensions.
Polymerase chain reaction - Wikipedia
PCR is a method to amplify a specific DNA sample rapidly by repeated cycles of heating and cooling. It is widely used in genetic testing, research and forensic science. Learn the principles, procedure and applications of PCR.
Learn how to use TaKaRa LA Taq DNA Polymerase for long range PCR (>15 kb fragments) with this user manual. Find information on product specifications, applications, PCR test, and patent notices.
End Point PCR Protocol for Long and Accurate DNA Amplification
Learn how to use AccuTaq LA and KlenTaq LA DNA Polymerase Mixes for PCR applications that require longer and more accurate amplification. Find optimal reagents, equipment, assay considerations and troubleshooting tips for end point PCR.
Polymerase Chain Reaction (PCR) Fact Sheet - National Human …
2020年8月17日 · Learn what polymerase chain reaction (PCR) is, how it works, and what it is used for in molecular and genetic analyses. PCR is a fast and inexpensive technique that copies small segments of DNA and is essential for many laboratory and clinical procedures.
Polymerase Chain Reaction (PCR) - StatPearls - NCBI Bookshelf
2023年3月6日 · The polymerase chain reaction (PCR) is a laboratory nucleic acid amplification technique used to denature and renature short segments of DNA using DNA polymerase I enzyme, an isolate from Thermus aquaticus, known as Taq polymerase.[1][2] In 1985, PCR was introduced by Mullis et al, who were later awarded the Nobel Prize for their work.[3]