Kuhner XC series Kuhner was the first company to manufacture incubator shakers with controlled CO 2 and humidity for the cultivation of mammalian cells. The «XC» series provides optimised …
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Therefore the orbitally shaken single-use bioreactor SB10-X has been developed and characterised. The determined scale-up factor kLa was used to perform a scale-up with 3 orbitally shaken bioreactors (culture volumes: 60 mL, 1.5 L and 10 L).
• Measures online OTR, CTR and RQ in up to 16 shake flasks • Modular design: choose between 4, 8 ,12 or 16 shake flasks • Zero footprint on the shaker tray as TOM is mounted above • Robust: Durable sensors and static electronics • Available for Kuhner ISF1-X, LT-X and Kuhner Kelvin+incubators • Various glass and single use flasks ...
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The graph shows that the aeration rate varies depending on the type of shaken bioreactor. To keep a constant value within the shaken bioreactors the user has to adjust the liquid volume or, if using the Tubespin, close a certain number of cap holes with sticky tape (De Jesus et al., 2004). ISF1-X, KUHNER, CH
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The key parameters that will impact the OTRmax of any shaken process include the: (1) osmolality of the solution, (2) shaking frequency, (3) filling volume, (4) shaker diameter, and …
Different types of shake flasks were placed in ani incubator (Kuhner Shaker, ISF1-XC, Switzerland) with controlled temperature, humidity and shaking speed. After one day the start weight of the shake flasks filled with water (30% of the nominal volume) were determined.