Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells using HSP90 Antibody. Cat. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. HSP90 Antibody detects endogenous levels of total HSP90 protein, alpha and beta isoforms.

By Western blot, this antibody detects an ~86 kDa protein representing HSP86 from HeLa cell lysate. Immunofluorescence staining of HSP86 in HeLa cells with PA3-013 yields a pattern consistent with cytoplasmic staining. Immunoprecipitation experiments with this antibody suggest that HSP86 exists primarily as homodimers in HeLa cells.

Western blot analysis of extracts from various cell lines using HSP90β Antibody. Cat. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. HSP90β Antibody detects endogenous levels of total HSP90β protein. It does not cross-react with HSP90α.

2020年7月26日 · HSP 90 Antibody (4F10) is a mouse monoclonal IgG2b antibody that detects HSP 90 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA).

Antibodies that detect HSP90 beta can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry and Peptide array. These antibodies target HSP90 beta in Human, Mouse, …

2019年1月26日 · HSP 90α/β Antibody (F-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects HSP 90 alpha/beta protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent ...

2025年2月8日 · Western blotting. The protein levels for HSP90, PINK1, BNIP3, GP91/NOX2, IRE1α, PERK, and β-actin were measured by Western blot. Briefly, normal cells and RKO were seeded in 10 cm cell culture dishes and allowed to attach overnight. Next, RKO cells were treated with AT13387 for 24 h. After treatment, cells were scraped, and protein was ...

By Western blot, this antibody detects a 90 kDa protein representing HSP90 from MCF7 cell extract. MA3-011 is more sensitive in Western blot procedures and should be used when maximum sensitivity is desired.