pTRIOZ-hIgG1 is designed for high-yield production of whole monoclonal antibodies (mAbs) with the use of a single plasmid. It consists of three cassettes encoding for the expression of the mAb heavy chain, light chain, and antibiotic selection with Zeocin®.

pFUSE-hIgG1-Fc are cloning plasmids for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the IgG1 heavy chain and the hinge region. pFUSE-hIgG1-Fc plasmids are now available with or without the IL2 signal sequence.

hIgG1-Fc (human): The Fc region comprises the CH2 and CH3 domains of the IgG heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.

Recombinant human IgG antibodies (hIgGs) completely devoid of binding to Fcγ receptors (FcγRs) and complement protein C1q, and thus with abolished immune effector functions, are of use for various therapeutic applications in order to reduce FcγR activation and Fc …

2015年6月1日 · Engineering of the constant Fc part of monoclonal human IgG1 (hIgG1) Abs is an approach to improve effector functions and clinical efficacy of next-generation IgG1-based therapeutics.

In this engineering system, four amino-acid mutations (T366W in the knob chain and T366S, L368A, Y407V in the hole chain) are made into a pair of different Fcs to preferentially form the heterodimer of the CH3 domains of human immunoglobulin γ1 (hIgG1) .

2015年6月1日 · Engineering of the constant Fc part of monoclonal human IgG1 (hIgG1) Abs is an approach to improve effector functions and clinical efficacy of next-generation IgG1-based therapeutics.

Sequence and structural alignment between mIgG1, mIgG2a, and hIgG1 indicated the two salt bridges previously described are well conserved, suggesting that the same mG1 KD strategy will likely be transferable to the mIgG2a. Thus, similar “K/D” mutations were designed for mIgG2a.

We have successfully developed a single-chain variable fragment (ScFv) combination of anti-CD3 ScFv and anti-CD138 ScFv with the hIgG1 Fc (hIgFc) sequence. The novel bispecific T-cell engager (BiTE) with an additional hIgFc (BiTE-hIgFc, STL001) can target T cells, natural killer cells, and multiple myeloma cells (RPMI-8226 or U266).

InvivoGen offers the 4 most popular human and murine IgG isotypes used in mAb production, optimized to ensure maximum production. pTRIOZ is utilized in-house to produce InvivoGen’s expanding mAb collection. The pTRIOZ collection of vectors are for high-yield production of recombinant monoclonal antibodies (mAb) using a single plasmid.