HLA region of Chromosome 6. The human leukocyte antigen (HLA) system is a complex of genes on chromosome 6 in humans that encode cell-surface proteins responsible for regulation of the immune system. [1] The HLA system is also known as the human version of the major histocompatibility complex (MHC) found in many animals. [2]

Here, we review each of the 6 HLA binding pockets of HLA class I (HLA-I) molecules. The binding specificity of pockets B and F are strong determinants of peptide binding and have been used to classify HLA into supertypes, a useful tool to predict peptide binding to a given HLA.

2021年11月1日 · Here, we review each of the 6 HLA binding pockets of HLA class I (HLA-I) molecules. The binding specificity of pockets B and F are strong determinants of peptide binding and have been used to classify HLA into supertypes, a useful tool to predict peptide binding to a …

Our optimized procedure to isolate HLA-DR peptides from low cell numbers included cell lysis, immunoprecipitation of HLA-DR, elution of HLA-DR bound peptides, desalting and peptide concentration, and identification by mass spectrometry followed by peptide filtering.

2023年10月10日 · Here, analysis of X-ray crystal structures within our curated database (HLA3DB) shows that pHLA complexes encompassing multiple HLA allotypes present a discrete set of peptide backbone...

2022年3月23日 · Human leukocyte antigen (HLA) can recognize and bind foreign peptides to present them to specialized immune cells, then initiate an immune response. Computational prediction of the peptide...

2010年3月10日 · Human leukocyte antigen (HLA) class I molecules present endogenous derived peptides of 8–12 amino acids (aa) in length to CD8 + cytotoxic T lymphocytes (CTL). 1 Most amino acid polymorphisms of different HLA class I molecules are located in the peptide-binding region (PBR) shaped by parts of the α1 and α2 domains and determine the characteristic...

A single amino acid substitution in HLA-B resulted in a significantly lower overlap in peptide-binding repertoire compared to that in HLA-A molecules in 15 out of 22 positions (68 %), suggesting that the peptide-binding groove of HLA-B molecules is …

2023年3月10日 · We demonstrate greater than twofold improved coverage of HLA immunopeptidomes relative to prior methods with up to 15,000 distinct HLA-I and HLA-II peptides from 4e7 cells.

2024年12月17日 · Here, we developed a convolution and attention-based model, CapHLA, trained with eluted ligand and binding affinity mass spectrometry data, to predict peptide presentation probability (PB) and binding affinities (BA) for HLA-I and HLA-II.