Hfr mapping - Columbia University
An example of an Hfr mapping experiment. Donor: Hfr strs serA--origin of transfer @ 97 min. Donates CCW. Recipient: F- pan-2, gal-9, trp-10, put-11. pan = pantothenate synthesis …
Hfr cell - Wikipedia
A high-frequency recombination cell (Hfr cell) (also called an Hfr strain) is a bacterium with a conjugative plasmid (for example, the F-factor) integrated into its chromosomal DNA. The integration of the plasmid into the cell's chromosome is through homologous recombination. A conjugative plasmid capable of chromosome integration is also called an episome (a segment of DNA that can …
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[3] Conjugational methods for mapping with Hfr and F-prime strains
1991年1月1日 · This chapter discusses conjugational methods for mapping with Hfr and F-prime strains. Conjugational mapping methods are particularly useful when the map location of a …
- 作者: Low Kb
- Publish Year: 1991
Hfr mapping of mutations in Bordetella pertussis that define a …
We report the development of techniques for the genetic mapping of point mutations in the bacterial pathogen Bordetella pertussis. A plasmid vector which is self-transmissible by …
- 作者: S Stibitz, N H Carbonetti
- DOI: 10.1128/jb.176.23.7260-7266.1994
- Publish Year: 1994
- Publication: J Bacteriol. 1994 Dec; 176(23): 7260-7266.
Hfr mapping - **Problem 2 ** You have done a series …
Draw a chromosome map showing all the genes, the position of the origin of each Hfr, the direction of each Hfr, and the distances between each marker. Use a directional arrowhead to indicate each origin of transfer.
Mapping in Bacteria • Three methods of Recombination in Bacteria: • Conjugation • Transformation • Transduction: Generalized transduction & Specialized transduction
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Gradtrans99 - Columbia University
Gradient of transmission mapping. A simplified method for Hfr mapping requiring only one time point. Breaking apart of mating pairs in Hfr cross is random and unrelated to time of mating.
orientation of Hfr fragments Overlap in transfer maps from different strains allow generation of a complete chromosomal map. Example • Four different Hfr strains of E. coli were mated to F- …
[34] Genetic mapping by Hfr mating - ScienceDirect
1971年1月1日 · Consider the mating of Hfr P4X6, which transfers, in order, proline (Pro), leucine (Leu), arginine (Arg), streptomycin (Sm), and histidine (His), with a female which requires …
Lecture 8: Gene mapping in bacteria. 1. Bacterial cultures. 2. Bacterial conjugation. 3. Mapping with Hfr strains. 4. Recombination in bacterial crosses. Escherichia coli. Theodor von …
