Supplementary test information for Antinuclear Antibody (ANA) With HEp-2 Substrate such as test interpretation, additional tests to consider, and other technical data.

Autoantibodies targeting antigens in the nuclear region in the HEp-2 cell line substrate using the indirect immunofluorescence assay (IFA) have traditionally been called antinuclear antibody (ANA).

Antinuclear antibodies (ANAs, also known as antinuclear factor or ANF) [2] are autoantibodies that bind to contents of the cell nucleus. In normal individuals, the immune system produces antibodies to foreign proteins (antigens) but not to human proteins (autoantigens).

Pattern on the antinuclear antibody-HEp-2 test is a critical parameter for discriminating antinuclear antibody-positive healthy individuals and patients with autoimmune rheumatic diseases Arthritis Rheum .

2022年4月25日 · Your blood sample is mixed with human cells (usually HEp-2 cells) fixed on a slide. If ANAs are present in your blood, they will bind to the nuclei of these cells. A fluorescent dye is then added, which attaches to the antibodies.

The indirect immunofluorescence assay (IIFA) on HEp-2 cells is widely used for detection of antinuclear antibodies (ANA). The dichotomous outcome, negative or positive, is integrated in diagnostic and classification criteria for several systemic ...

Antinuclear antibody (ANA) testing is used in the diagnostic evaluation of various autoimmune diseases, including connective tissue diseases such as systemic lupus erythematosus (SLE), Sjögren syndrome, and systemic sclerosis (SSc).

The HEp-2 cell line contains HeLa marker chromosomes and were derived via HeLa contamination. This line was originally thought to be derived from an epidermoid carcinoma of the larynx but was subsequently found – based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting – to have been established via HeLa cell contamination.

2018年4月29日 · HEp-2 cell line offers the possibility for detection of more than hundred different autoantibodies to nuclear and cytoplasmic autoantigens. The effects of ANA detection with HEp-2 line was the basis of American College of Rheumatology recommend to use of IIF method as the gold standard for ANA detection (screening) [ 5 , 6 ].

Hep-2/Hep2 cells are invaluable for researchers engaged in the analysis of autoantibodies, and they are currently one of the most common substrates for antinuclear antibody detection by immunofluorescence [6, 7]. The Hep-2 cell line was first specifically described …