To assess the function of the Gns1 gene in disease resistance, we characterized transgenic rice plants constitutively expressing the Gns1 gene. The introduced Gns1 gene was driven by the CaMV 35S promoter and its products were found in the apoplast and accumulated in up to 0.1% of total soluble protein in leaves.

Feb 27, 2003 · RNA和蛋白质凝胶印迹分析表明原始病或黑暗处理诱导了Gns1基因的表达。 为了评估Gns1基因在抗病性中的功能，我们表征了组成型表达Gns1基因的转基因水稻植株。

该基因（Gns1）在发芽的种子，芽，叶，穗和愈伤组织中的表达水平相对较低，而在根中则表达水平较高。 根中的表达似乎是组成性的。 当用乙烯，细胞分裂素，水杨酸和源自病原菌米氏菌或非病原性酿酒酵母的真菌引发剂处理时，芽表达的Gns1含量要高得多。

The GNS1 gene product is required for the synthesis of 1,3-beta-glucan in vitro, since mutations in this gene result in exhibit an 80 to 90% reduction in 1,3-beta-glucan synthase specific activity. gns1 mutant strains display a pleiotropic phenotype including resistance to a pneumocandin B0 analog (L-733,560), slow growth, and mating and ...

Nov 26, 1998 · Gns1 of rice is moderately expressed in roots, young shoots, callus and immature seed, but there is very little expression in seedling caryopsis or coleoptile (Table 4). The different hybridization probes used may explain the differences between these expression results and those reported previously for Gns1 ( Simmons et al., 1992 ).

gns1突变菌株表现出多效性表型，包括对肺炎链菌素B0类似物（L-733,560）的抗性，生长缓慢以及交配和孢子形成缺陷。gns1-1突变被遗传定位到染色体III上MAT基因座的1.35厘摩范围内。

The gns1-1 mutation was genetically mapped to within 1.35 centimorgans from the MAT locus on chromosome III. The wild-type GNS1 gene was isolated by complementing the pneumocandin resistance phenotype of the gns1-1 mutation and by hybridization with a chromosome III-derived sequence being used as a probe.

Oct 4, 2023 · GNS1/SUR4 family Members of this family are involved in long chain fatty acid elongation systems that produce the 26-carbon precursors for ceramide and sphingolipid synthesis. Predicted to be integral membrane proteins, in eukaryotes they are probably located on the endoplasmic reticulum.

The Gns1 gene of rice (Oryza sativa L. japonica) encodes 1,3;1,4-beta glucanase (EC 3.2.1.73), which hydrolyzes 1,3;1,4-beta-glucosidic linkages on 1,3;1,4-beta-glucan, an important component of cell walls in the Poaceae family. RNA and protein gel blot analyses demonstrated that blast disease or da …

Sep 18, 2024 · Gene provides a unified query environment for genes defined by sequence and/or in NCBI's Map Viewer.