2007年6月1日 · GEFs and GAPs are multidomain proteins that are regulated by extracellular signals and localized cues that control cellular events in time and space. Recent evidence …

Guanine nucleotide exchange factors (GEFs) are proteins or protein domains involved in the activation of small GTPases. Small GTPases act as molecular switches in intracellular …

2007年6月1日 · GEFs and GAPs are multidomain proteins that are regulated by extracellular signals and localized cues that control cellular events in time and space. Recent evidence …

Describe how guanine nucleotide exchange factors (GEFs) promote the release of GDP and facilitate GTP binding, reactivating the small G proteins. Identify the key structural elements of …

2010年11月24日 · Guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs) that control the GDP–GTP cycling of specific members of the Ras superfamily have …

GEFs and GAPs are multidomain proteins that are regulated by extracellular signals and local-ized cues that control cellular events in time and space. Recent evidence suggests that these …

This unit describes (1) detailed protocols for the expression and the purification of small GTPases and the catalytic domains of GEFs and GAPs; (2) preparation of nucleotide-free and …

Three decades of intense Ras research have disclosed various families of guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs) as the two principal …

2014年1月1日 · GEFs and GAPs have themselves sophisticated mechanisms that regulate their activities, whose biochemical and structural analysis are key issues for future investigations.

Rabs are GTP-binding proteins with conserved functions in membrane trafficking. They are regulated by a diverse group of structurally unrelated GDP-GTP exchange factors (GEFs), and …