The 293 cell line is a permanent line established from primary embryonic human kidney transformed with human adenovirus type 5 DNA. The genes encoded by the E1 region of adenovirus (E1a and E1b) are expressed in these cells and participate in transactivation of viral promoters, allowing these cells to produce high levels of protein.
HEK293 cell line express eGFP fluorescent reporter - GenTarget Inc
Transformed from adherence-enhanced HEK293 cells, the cell line expresses the enhanced GFP under CMV promoter. The cell line carries the Blasticidin resistance, see the integrated expression cassette scheme below.
293-GFP细胞_人胚肾细胞（绿色荧光） - 武汉尚恩生物技术有限公司
293 [hek-293]细胞可以作为人类腺病毒载体扩增的宿主，可表达异常的玻连蛋白的细胞表面受体，由整合素β1亚单位和玻连蛋白受体α-v亚单位组成。293 [hek-293]细胞可用于工业生物技术和毒理学研究，功效测试和病毒测试中也有应用。
HEK-293-GFP-Puro稳转株-镇江维根生物科技有限公司
英文名称. HEK-293-GFP-Puro polyclonal stable cell line. 产品货号. VGC-0206-001P. 中文名称. GFP 标记的 HEK-293 稳转株. Lot number. HEK-293-10A1P. 细胞规格. 复苏细胞 (T25细胞瓶) 或1 × 10 6 细胞冻存细胞. 细胞形态
Puro-GFP HEK-293 Cell Line - Genomeditech
基于HEK-293细胞，使用慢病毒技术构建的稳定表达Puro-GFP的荧光对照细胞系。
293、293T、293FT 、293A的区别和培养攻略 - BIOON
HEK-293人胚肾细胞 ：是用 5 型腺病毒 75 株系转化，含有 Ad5 E1 区的人胚肾亚三倍体细胞系，是一种 E1 区缺陷互补细胞系。它是加拿大 McMaster University 的 F.L.Graham 与 J.S.Miley 于 1976 年用 DNA 转染技术构建而成。 该细胞为人类腺病毒载体扩增的宿主。
GFP HEK-293 Stable Cell Line - Abeomics
GFP/HEK293 Stable Cell Line is a stably transfected HEK293 cell line which expresses enhanced green fluorescent protein (eGFP). Cells should be grown at 37 C with 5% CO 2 using DMEM medium (w/ L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate) supplemented with 10% heat-inactivated FBS and 1% Pen/Strep, plus 3 µ g/ml of Puromycin.
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293/GFP Cell Line
The 293 cell line is a permanent line established from primary embryonic human kidney transformed with human adenovirus type 5 DNA. The genes encoded by the E1 region of adenovirus (E1a and E1b) are expressed in these cells and participate in transactivation of viral promoters, allowing these cells to produce high levels of protein.
HEK293, HEK293-GFP Cells, HEK Cells - Molecular Devices
HEK293 cells are easy to grow and transfect, so they have been used extensively in transfection-based experiments, protein expression, and vaccine production. We imaged HEK293 cells lentivirally transformed with Green Fluorescent Protein (GFP; GenTarget Inc.) to showcase analysis of cell confluence on the SpectraMax MiniMax 300 Imaging Cytometer.
流式检测GFP 操作步骤可调整优化，对于本实验，推荐Puro-GFP HEK-293 Cell Line细胞量为2 × 105 cells/管。操作步骤如下： a) 实验前，需等待细胞生长速率稳定，约需要3-5 d。 b) 实验当天，1000 rpm离心5 min，去除上清，用300 μL PBS重悬。 c) 立即上机检测。 d) 验证结果。