Feb 7, 2018 · The C1™ system supports DNA sequencing, mRNA sequencing, targeted gene expression, miRNA analysis and additional applications through the C1 Open App IFC (integrated fluidic circuit). Dow...

This unit describes a procedure utilizing an automated microfluidic platform, the Fluidigm C1 system, to simultaneously isolate dozens of single cells in a size- and shape-dependent manner. The microfluidic platform processes cells in individual nanoliter-scale reactions to convert their contents into double-stranded cDNA.

The C1™ system takes an entirely new approach, based on innovative Fluidigm® microfluidic technology that enables you to rapidly and reliably isolate, process, and profile individual cells for genomic analysis.

SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System, IFCs User Manual

C1 is an automated system that carefully isolates single cells into individual reaction chambers in the exclusive Fluidigm integrated fluidic circuit (IFC). C1 lets you prepare single-cell templates for mRNA sequencing, DNA sequencing, epigenetics or miRNA expression.

The C1 system isolates single cells into individual reaction chambers in the exclusive Fluidigm integrated fluidic circuit (IFC). The optically clear IFC lets you automatically stain captured cells and examine them by microscopy for viability, surface markers, or reporter genes.

Our data show that the C1 Fluidigm platform can be used for single-cell RNA sequencing, allowing identification of all islet cell types. We also demonstrate that half of the cells were damaged during the capture process, resulting in markedly altered gene expression patterns.

The C1 system isolates single cells into individual reaction chambers in Fludigm’s integrated fluidic circuit (IFC). The optically clear IFC allows for automated staining of captured cells and examination by microscopy for viability, surface markers or reporter genes.

The NGSC has recently purchased a Fluidigm C1 system that greatly eases sequencing and other measurements of single cells. The most common application is mRNA RNA-Seq libraries, but whole genome, targeted sequencing, and other custom applications are possible.

The development of single-cell RNA sequencing (RNA-seq) enabled a high-resolution and unbiased analysis of cell transcriptomes. This unit describes a procedure utilizing an automated microfluidic platform, the Fluidigm C1 system, to simultaneously isolate dozens of single cells in a size- and shape-dependent manner.