FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. 22,23 FITC-dextran has been used to study plant cell wall porosity 24 and capillary permeability. 25,26 Plasma proteins have been shown not to bind to FITC-dextran. 26

FITC-Dextran, also known as fluorescein isothiocyanate-labeled dextran, is a versatile molecular marker extensively applied in cell and tissue research, encompassing biochemical investigations. This compound is the result of linking the fluorescent FITC dye with dextran, a polysaccharide composed of branching glucose molecules of varied lengths

Fluorescein isothiocyanate (FITC)-dextrans are synthesised by coupling fluoresceinyl isothiocyanate (5-isomer) to selected dextran fractions. All the batches are checked for molecular weight, degree of substitution, loss on drying and free FITC. TdB Labs produces FITC-dextrans from 4 kDa to 2000 kDa that are supplied as a yellow to orange powder.

2019年8月21日 · FITC-Dextran (MW 10000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 10000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis.

2017年4月10日 · A detailed protocol is presented for assessing the integrity of the BBB by transcardial perfusion of a 10,000 Da FITC-labeled dextran molecule and its visualization to determine the degree of extravasation from brain microvessels. © 2017 by John Wiley & Sons, Inc. Keywords: FITC-dextran extravasation; blood-brain barrier; brain capillaries.

FITC-Dextran (MW 4000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 4000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis.

FITC-dextran visualization requires a 488 nm excitation wavelength laser and an emission collection spectrum of 504–556 nm. The images can be captured at 4 μm z steps. Use the same Gain, Offset and exposure settings for all images captured within one experiment.

Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals. FITC-dextran is used extensively in microcirculation and cell permeability research utilizing microfluorimetry. FITC-dextran has been used to study plant …

This protocol describes the use of the Fluorescein isothiocyanate (FITC)–dextran trans-epithelial permeability assay to quantify the effects of chemicals on epithelial cell monolayer permeability. The attached methods document is a formal version of the information included here.

Fluorescein isothiocyanate–dextran (FITC-Dextran 70) is use as a fluorescent probe to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery.