FBXW7/GSK3β-mediated proline-rich 11 degradation promotes …
2025年2月3日 · FBXW7 promotes ubiquitination and degradation of PRR11. (A) Comparison of conserved PRR11 sequences with known CPD motifs recognized by FBXW7. (B) Western blot …
FBXW7 antibody (55290-1-AP) | Proteintech - ptglab
55290-1-AP targets FBXW7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. …
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FBXW7 modulates cellular stress response and metastatic ... - Nature
2015年2月27日 · HA-tagged FBXW7α was immunoprecipitated (IP) from cell extracts with anti-HA resin, followed by immunoblotting as indicated. The left panel shows inputs.
FBXW7 tumor suppressor regulation by dualspecificity tyrosine …
2023年3月18日 · FBXW7 is a main tumor suppressor due to its ability to control proteasome-mediated degradation of several oncoproteins such as c-Jun, c-Myc, Cyclin E1, mTOR, and …
The FBXW7‐RPAP2 Axis Controls the Growth of Hepatocellular …
2025年2月11日 · A follow-up immunoprecipitation (IP) assay confirmed the interaction of endogenous RPAP2 with wild-type FBXW7 but not with the FBXW7 R479L mutant in HEK293 …
Here we report that LSD1 directly binds to FBXW7 to destabilize FBXW7 independent of its demethylase activity. Specifically, LSD1 is a pseudosubstrate of FBXW7 and LSD1–FBXW7 …
The FBXW7–KMT2 axis in cancer-associated fibroblasts controls
(D) HEK293 cells were transfected with indicated amounts of HA-FBXW7 for 48 h, followed by IP with RbBP5 Ab and IB. (E and F) HEK293 cells were transfected with indicated siRNA for 48 h …
孙毅/谢传明合作团队揭示E3连接酶FBXW7的抑癌新机制_光谱网
5 天之前 · fbxw7是一种抑制肿瘤生长的scf e3泛素连接酶。 在大多数情况下，FBXW7识别磷酸化底物并促进它的泛素化降解【1】。 目前报道，FBXW7主要通过泛素化降解几个关键促癌蛋 …
FBXW7-mediated ERK3 degradation regulates the proliferation of …
2022年1月12日 · IP results obtained using HA-FBXW7 and these expression vectors showed that mutation of ERK3 Thr417 and Thr421 to alanine (Myc-ERK3-FL-mtPDM2) dramatically …
FBXW7 Directly Ubiquitinates and Degrades CTNNB1 Mediating …
Co-IP assay and immunofluorescence staining showed cytoplasmic colocalization of CTNNB1 and FBXW7 proteins in EC cells (Fig. 7A-C). Western blotting experiments revealed a marked …