The Cre-lox and FLP-FRT systems - The Jackson Laboratory
Two of the most exciting and versatile genetic tools designed in the last 30 years are the Cre-lox and FLP-FRT technologies. Both allow the location and timing of gene expression to be closely regulated. This article briefly outlines how the two systems work and can be used in …
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View …
Cre-mediated possibilities for site specific (and often cell type specific) control of DNA recombination and gene expression can be advanced by the coordinated use of fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression have been developed.
Flp-In System: For Generating Constitutive Expression Cell Lines
The Invitrogen™ Flp-In™ System allows integration and expression of your gene of interest in mammalian cells at a specific genomic location. The Flp-In System involves introduction of a Flp Recombination Target (FRT) site into the genome of the mammalian cell line of choice.
Going beyond Cre-lox - The Jackson Laboratory
Most mouse geneticists who work with Cre-lox mice probably are familiar with the FLP-FRT system. Like Cre, FLP is a tyrosine recombinase, but it originates from the yeast Saccharomyces cerevisiae, rather than from bacteriophage.
Site-specific chromosomal gene insertion: Flp recombinase
Dec 19, 2017 · Here we established a recombinase-mediated cassette exchange (RMCE) protocol for the fast and effective, drug-free isolation of recombinant cells. Distinct fluorescent protein patterns...
Application of the FLP/FRT system for conditional gene deletion …
To complement the many methods used to control gene expression in yeast, we have established a conditional gene deletion system by using the FLP/FRT system on yeast vectors to conditionally delete specific yeast genes.
Flp-In™ Complete System - Thermo Fisher Scientific
Our Flp-In™ Complete System allows integration and expression of your gene of interest in mammalian cells at a specific genomic location. The Flp-In System involves introduction of a Flp Recombination Target (FRT) site into the genome of the mammalian cell line of choice.
Applications of the Saccharomyces cerevisiae Flp-FRT system …
The Flp-FRT site-specific recombination system from Saccharomyces cerevisiae is a powerful and efficient tool for high-throughput genetic analysis of bacteria in the postgenomic era. This review highlights the features of the Flp-FRT system, describes current bacterial genetic methods incorporating …
Cre/loxP, Flp/FRT Systems and Pluripotent Stem Cell Lines
Jan 1, 2012 · This chapter shall provide a brief historical perspective on the discovery and early development of the Cre/loxP and FRT/FLP systems citing key studies that paved the way for the application of these site-specific recombination technologies to …
A broad-host-range Flp-FRT recombination system for site …
May 28, 1998 · This paper describes an efficient procedure for isolation of unmarked mutant alleles in the P. aeruginosa chromosome, utilizing a combination of versatile gene replacement vectors and a bhr version of the S. cerevisiae Flp-FRT recombination system.
- Some results have been removed