Apr 1, 2003 · E2F activity is crucial for the G1/S transition and DNA replication in mammalian cells. The retinoblastoma (pRB) family of proteins is the primary negative regulator of E2F. Recent findings have begun to clarify distinct roles for E2F family members during cell cycle progression and have considerably broadened our understanding of E2F ...

Cyclin E-CDK2负责完成RB1的磷酸化，通过释放更多的E2F，促进细胞进入S期所需的蛋白质的合成。 在G1期的发展过程中，一些E2F转录生成的蛋白在DNA复制起点聚集并形成 复制前复合体 ，由ORC1-6、CDC6、CDT1和MCM2-7 DNA解旋酶组成。

细胞周期蛋白依赖性激酶 (CDK)-RB-E2F 调控轴组成了驱动细胞周期进程、决定基因组复制的时间和准确性、并确保遗传物质准确传递的核心转录机制。 该调控轴的最终效应子是编码转录激活因子和抑制因子的8个不同 E2F 基因家族的成员（E2F1-8）。 E2F 转录活性在整个细胞周期中通过转录和翻译调控、翻译后修饰、蛋白质降解、与辅因子的结合和亚细胞定位等过程受到严格调控。 该调控轴的一个或多个关键成分 (CDKs, cyclins, CDK inhibitors and the RB family of proteins) …

在 g1 期的未定型细胞中，低磷酸化的 rb 结合到 e2f-dp1 转录因子，并与 hdac 一起形成一个抑制复合体，从而抑制下游的关键转录活动。 明确进入 S 期是通过周期素 D-CDK4/6 和 周期素 E-CDK2 连续磷酸化 Rb 实现的，前两者可将 HDAC-抑制复合体分离，并允许进行 DNA 复制 ...

Oct 15, 2020 · The Cdk-Rb-E2F pathway integrates external and internal signals to control progression at the G1/S transition of the mammalian cell cycle. Alterations in this pathway are found in most human cancers, and specific cyclin-dependent kinase Cdk4/6 inhibitors are approved or in clinical trials for the treatment of diverse cancers.

We find that E2F6 associates specifically with the E2F target genes that are activated at G1/S; this interaction occurs during S phase of the cell cycle. In sharp contrast, E2F6 does not bind to E2F-regulated genes activated at G2/M. In the absence of E2F6, E2F4 can bind to the G1/S-regulated promoters and compensate for loss of E2F6 function.

Jul 23, 2013 · This classic paradigm of the role of activator E2F proteins in inducing G1–S gene expression and driving cell cycle entry derives mainly from studies using cultured cells and flies.

E2F activity is crucial for the G1/S transition and DNA replication in mammalian cells. The retinoblastoma (pRB) family of proteins is the primary negative regulator of E2F. Recent findings have begun to clarify distinct roles for E2F family members during …

Nov 24, 2004 · The ability of B-Myb to interact with the cdc2 promoter is dependent on an intact E2F binding site. These results thus point to a role for E2Fs, together with B-Myb, which is an E2F-regulated gene expressed at G1/S, in linking the regulation of genes at G1/S and G2/M.

g1/s 检查点可控制细胞通过限制点 (r) 进入 dna 合成 s 期的进程。 在 G1 阶段，抑癌基因 Rb 结合并抑制转录因子 E2F。 在 G1 末期被周期素依赖性激酶 (CDK) 磷酸化的 Rb 会诱使 Rb 分裂，并允许 E2F 介导的 S 期促进基因的转录。