The original variant occurs naturally in the coral genus Discosoma, and is named DsRed. Several new variants have been developed using directed mutagenesis [1] which fluoresce orange, red, and far-red. [2]

DsRed is a basic (constitutively fluorescent) red fluorescent protein published in 1999, derived from Discosoma sp..

2000年10月24日 · DsRed, a brilliantly red fluorescent protein, was recently cloned from Discosoma coral by homology to the green fluorescent protein (GFP) from the jellyfish Aequorea. A core question in the biochemistry of DsRed is the mechanism by which the GFP-like 475-nm excitation and 500-nm emission maxima of immature DsRed are red-shifted to the 558-nm ...

Since the discovery of the first red fluorescent protein (RFP), named DsRed, 12 years ago, a wide pallet of red-shifted fluorescent proteins has been cloned and biotechnologically developed into monomeric fluorescent probes for optical microscopy. ...

2002年1月1日 · DsRed.T3 matures rapidly, and the purified protein is nearly as bright as mature wild-type DsRed , making this variant well suited to single-color imaging of red fluorescence.

The crystal structure of DsRed, a red fluorescent protein from a corallimorpharian, has been determined at 2.0-Å resolution by multiple-wavelength anomalous dispersion and crystallographic refinement.

We describe the utilization of a red fluorescent protein (DsRed) as an in vivo marker for Saccharomyces cerevisiae. Clones expressing red and/or green fluorescent proteins with both cytoplasmic and nuclear localization were obtained.

dsRed Express was developed to accelerate chromophore formation. Through key mutations, this variant fluoresces within hours rather than days. One improvement was stabilizing intermediate oxidation states, expediting the transition from the green-emitting precursor to the red fluorescent form. Additionally, dsRed Express exhibits reduced ...

The red fluorescent protein DsRed has spectral properties that are ideal for dual-color experiments with green fluorescent protein (GFP). But wild-type DsRed has several drawbacks, including slow chromophore maturation and poor solubility.

DsRed is an interesting marker protein for use in plants because of its red-shifted wavelength spectrum that will avoid damaging cells and tissues by excitation light. In this report, we show that DsRed is an excellent marker in higher plants in spite of the interfering red autofluorescence of chlorophyll, which can be eliminated by using the ...