Drosha is a Class 2 ribonuclease III enzyme [5] that in humans is encoded by the DROSHA (formerly RNASEN) gene. [6][7][8] It is the primary nuclease that executes the initiation step of miRNA processing in the nucleus. It works closely with DGCR8 and in correlation with Dicer.

2024年12月24日 · DROSHA (Drosha Ribonuclease III) is a Protein Coding gene. Diseases associated with DROSHA include Hereditary Breast Ovarian Cancer Syndrome and Lynch Syndrome 5. Among its related pathways are Formation of HIV elongation complex in the absence of HIV Tat and Translational Control.

Drosha is the catalytic center of the microprocessor and has two RNase-III domains that make up a catalytic site with each domain making one of the two cuts necessary to excise the pre-miRNA. Drosha excises the pre-miRNA from the pri-miRNA by cutting at the base of the dsRNA stem–loop of the pre-miRNA [82].

2016年4月29日 · A recent study by Kwon et al. in Cell reveals the structure of a DROSHA construct in complex with the C-terminal region of DGCR8, thereby unveiling the topology and interactions between...

2016年1月14日 · The heretofore-elusive structure of DROSHA provides insights into how nascent microRNAs are recognized and cleaved in their initial processing step and reveals an unexpected similarity between DROSHA and DICER, the enzyme …

2012年7月20日 · DROSHA is a nuclear RNase III enzyme responsible for cleaving primary microRNAs (miRNAs) into precursor miRNAs and thus is essential for the biogenesis of canonical miRNAs.

2016年1月14日 · MicroRNA maturation is initiated by RNase III DROSHA that cleaves the stem loop of primary microRNA. DROSHA functions together with its cofactor DGCR8 in a heterotrimeric complex known as Microprocessor. Here, we report the X-ray structure of DROSHA in complex with the C-terminal helix of DGCR8.

2018年2月7日 · Depletion of Drosha significantly reduces DNA repair by both homologous recombination (HR) and non-homologous end joining (NHEJ). Drosha is required within minutes of break induction,...

2024年7月30日 · Drosha, a key player in gene regulation, is an endoribonuclease enzyme responsible for initiating the processing of primary microRNA transcripts (pri-miRNAs) into precursor miRNAs (pre-miRNAs) in the nucleus.

2013年12月26日 · Drosha is the main RNase III-like enzyme involved in the process of microRNA (miRNA) biogenesis in the nucleus. Using whole-genome ChIP-on-chip analysis, we demonstrate that, in addition to miRNA sequences, Drosha specifically binds promoter-proximal regions of many human genes in a transcription-de …