Disruption mutant has reduced ability to catabolize D-xylonic acid. YjhG-yagF double mutant cannot use D-xylonate as the sole source of carbon. 1 publication

2018年1月16日 · In this paper, we describe the first crystal structure of a D-xylonate dehydratase at 2.7 Å resolution and compare it with the two other known crystal structures (PDB codes: 2GP4 and 5J84)...

D-xylonate dehydratase YjhG from Escherichia coli can convert D-xylonate into 2-keto-3-deoxy- D-xylonate (KDX), and is a key enzyme in the biosynthesis of 1,2,4-butanetriol and other chemicals....

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D-xylonate dehydratase YjhG from E. coli was purified as His 6 -tagged fusion protein to study its enzymatic characteristics in vitro. What's more, protein sequence alignment revealed that YjhG could be a novel member of IlvD/EDD family. These results will benefit applications of YjhG protein in biosynthesis of related chemicals.

D-xylonate dehydratase YjhG from Escherichia coli can convert D-xylonate into 2-keto-3-deoxy- D-xylonate (KDX), and is a key enzyme in the biosynthesis of 1,2,4-butanetriol and other chemicals.

2015年6月17日 · D-xylonate dehydratase YjhG from Escherichia coli can convert D-xylonate into 2-keto-3-deoxy- D-xylonate (KDX), and is a key enzyme in the biosynthesis of 1,2,4-butanetriol and other chemicals.

2024年10月11日 · Gene ID: 95405348, updated on 11-Oct-2024. YjhG/YagF family D-xylonate dehydratase. Gene provides a unified query environment for genes defined by sequence …

2012年7月1日 · The endogenous d -xylonic catabolic pathway in E. coli W3110 was previously reported by Frost (2008). Based on this study, two d -xylonic acid dehydratase genes (yagF and yjhG) were disrupted to prevent d -xylonic acid consumption. All of the gene disruptions were confirmed by PCR (Supplementary Fig. 2).

2015年7月24日 · D-xylonate dehydratase YjhG from E. coli was purified as His 6 -tagged fusion protein to study its enzymatic characteristics in vitro. What's more, protein sequence alignment revealed that YjhG could be a novel member of IlvD/EDD family. These results will benefit applications of YjhG protein in biosynthesis of related chemicals.