Dihydroorotate dehydrogenase (DHODH) is an enzyme that in humans is encoded by the DHODH gene on chromosome 16. The protein encoded by this gene catalyzes the fourth enzymatic step, the ubiquinone -mediated oxidation of dihydroorotate to orotate, in de …

Dihydroorotate dehydrogenase (DHODH) is a ubiquitous enzyme located within the inner membrane of the mitochondria. Using the cofactor ubiquinone, DHODH catalyzes the fourth step of de novo pyrimidine synthesis: the conversion of dihydroorotate to orotate.

DHODH catalyses oxidation of DHO into orotate by transferring electrons to the respiratory molecule ubiquinone through an enzyme-bound redox cofactor, FMN . Thus DHODH relies on ubiquinone, thereby forming a functional link between the …

2021年5月10日 · DHODH, a key enzyme in the de novo pyrimidine synthesis pathway, plays a crucial role in tumorigenesis via influencing pyrimidine metabolism and mitochondria function. Inhibiting DHODH has gradually become a hit spot in malignancy therapy.

The DHODH gene provides instructions for making an enzyme called dihydroorotate dehydrogenase. This enzyme is involved in producing pyrimidines, which are building blocks of DNA, its chemical cousin RNA, and molecules such as ATP and GTP that serve as energy sources in the cell.

Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme of the pyrimidine de novo pathway and it catalyzes the conversion of dihydroorotate to orotate. In most eukaryotes, DHODH is localized in mitochondria and the electrons are transferred directly to …

2017年10月15日 · DHODHs are extensively exploited as potential drug targets across rheumatology, oncology, and infectious diseases and are emerging as excellent models to study the evolution of enzymatic catalysis.

Here, we show that apoptosis in human colon carcinoma cells induced by the mitochondrial respiratory chain complex III inhibition can be prevented by supplementation with uridine or orotate (products of the reaction catalyzed by DHODH) rather …

2010年6月3日 · Dihydroorotate dehydrogenase (DHODH) is an enzyme of the de novo pyrimidine synthesis pathway that provides nucleotides for RNA/DNA synthesis essential for proliferation. In mammalian cells, DHODH is localized in mitochondria, linked to the respiratory chain via the coenzyme Q pool.

In this study, we demonstrated that two specific DHODH enzyme inhibitors, brequinar (BQR) and leflunomide (LFM) robustly inhibited rotavirus replication in conventional human intestinal Caco2 cell line as well as in human primary intestinal organoids.