2011年1月1日 · Acquisition of syphilis through occupational exposures, including contact with specimens collected for darkfield microscopy, has been reported. Proper specimen collection and handling is critical for optimizing the sensitivity of darkfield testing.

Syphilis is a multisystem infection caused by the spirochete Treponema pallidum. Currently, cases of possible syphilis are commonly investigated using the treponemal serological tests T. pallidum IgG chemiluminescence immunoassay (CLIA) and the T. pallidum particle agglutination (TPPA).

The diagnosis of syphilis may involve dark-field microscopy of skin lesions but most often requires screening with a nontreponemal test and confirmation with a treponemal-specific test....

For dark-field microscopy, the fluid should be collected on a slide, covered with a coverslip and examined within 20 min. For direct fluorescent antibody testing, a smear should be made on a slide and then air dried.

Treponema pallidum is a thin, delicate, tightly wound spirochaete that cannot be seen in Gram-stained smears. So, dark-field microscopy is used to demonstrate the presence of motile Treponema pallidum in lesions or aspirates in early-stage (primary or secondary) syphilis.

Demonstration of Treponema pallidum under the dark ground microscope is a rapid onsite method for diagnosis in early syphilis. The steps involve obtaining patient consent, specimen collection from an infective lesion of early syphilis, the adjustments of dark field microscope, and identification of treponemes by virtue of their characteristic ...

“A diagnosis of syphilis is confirmed by using dark field microscopy to demonstrate T. pallidum in material from suspected lesions, or regional lymph nodes (Creighton, 1990). A positive dark …

Dark-field microscopy is used to demonstrate the presence of motile Treponema pallidum in lesions or aspirates in early-stage (primary or secondary) syphilis before healing lesions. It is a …

These include dark-field microscopy, PCR, and direct fluorescent antibody testing for T pallidum. In some cases, these tests may allow the diagnosis of syphilis prior to a serologic response.

2022年1月1日 · DFM allows primary syphilis early diagnosis, even without serological test. If no direct detection methods are available, for patients without history of syphilis, TPPA could help to diagnose primary syphilis.