Blue intensity matters for cell cycle profiling in fluorescence DAPI ...
2017年3月6日 · Moreover, the accessibility to DNA fluorochromes that bind stoichiometrically to DNA (eg, DAPI) allows the direct quantitative estimation of the DNA content in a biological …
Observing Mitosis with Fluorescence Microscopy - National MagLab
2015年11月13日 · Mitosis, a phenomenon observed in all higher eukaryotes, is the mechanism that allows the nuclei of cells to split and provide each daughter cell with a complete set of …
Cell cycle staging of individual cells by fluorescence microscopy
On the basis of the DAPI integrated intensity values that define distinct cell cycle phases, as obtained by setting gates on the cell cycle histograms in the following protocol steps, these …
A Framework for Image-Based Classification of Mitotic Cells in ...
DAPI staining alone is insufficient for recognizing cells undergoing cytokinesis, and it can variably segment anaphase cells as one or two distinct nuclear objects per cell, depending upon the …
Stages of Mitosis in Naegleria Revealed by Staining for a-Tubulin ...
Mitosis in the amebo-flagellate Naegleria pringsheimi is acentrosomal and closed (the nuclear membrane does not break down). The large central nucleolus, which occupies about 20% of …
Replication stress activates DNA repair synthesis in mitosis
2015年12月2日 · Our findings reveal that mitotic DNA synthesis is detectable at sites of DAPI-negative chromosomal regions, suggesting that these cytogenetically defined loci comprise …
For mitotic index experiments, fixed cells were imaged using a Cytation C10 configured with a DAPI and TRITC LED cubes using a 4x objective. The DAPI cube is configured with a 377/50 …
D-MAINS: A Deep-Learning Model for the Label-Free Detection of Mitosis …
2024年6月8日 · Mitosis can be characterized by DAPI staining of the nucleus; chromosome condensation is a hallmark feature of a cell undergoing mitosis . For senescence, the most …
Mitotic index determination using microscopic fluorescence …
The metaphase/Anaphase index (M/AI) determination in the same sample was carried out by the microscopic examination through counting the proportion of DAPI-stained cells in mitosis.
Analysis of DAPI-stained HEK293 cells in the different sub-phases …
Download scientific diagram | Analysis of DAPI-stained HEK293 cells in the different sub-phases of mitosis, including bright-field, fluorescence and MCARS (CH 2 , CH 3 ) imaging.