In the burgeoning field of epigenetics, there are several methods available to determine the methylation status of DNA samples. However, choosing the method that is best suited to answering a particular biological question still proves to be a difficult task.

In this practical guide to the measurement and analysis of DNA methylation, the goal is to convey basic concepts in DNA methylation biology and explore genome-scale bisulfite sequencing as the current gold standard for assessment of DNA methylation.

The present study will serve as an introduction to methods for DNA methylation analysis. This review provides an overview of the major profiling approaches, with a focus on the recent and promising genome-wide methodologies.

Profiling DNA methylation across the genome is critical to understanding the influence of methylation in normal biology and diseases including cancer.

2012年9月18日 · Recent technological advances make it possible to map DNA methylation in essentially any cell type, tissue or organism. Computational methods and software tools are essential for processing,...

What is DNA methylation analysis? DNA methylation analysis allows scientists to gain valuable insight into gene regulation and identify potential biomarkers. Aberrant DNA methylation has been implicated in many disease processes, including cancer, obesity, and addiction.

In this review, we discuss the different DNA methylation analysis technologies primarily based on the initial treatments of DNA samples: bisulfite conversion, endonuclease digestion and affinity enrichment, involving methodology evolution, principles, applications, and their relative merits.

2011年10月24日 · DNA methylation is a biochemical process where a DNA base, usually cytosine, is enzymatically methylated at the 5-carbon position. An epigenetic modification associated with gene regulation, DNA methylation is of …

2010年2月2日 · There are three different initial treatments of DNA that can be used to reveal DNA methylation: endonuclease digestion, affinity enrichment and bisulphite conversion. The implementation of...

NEB offers a range of solutions for investigating DNA methylation. Enzymatic conversion is highly recommended over conventional bisulfite conversion to generate more accurate and sensitive 5mC results with next generation sequencing or PCR.