动态光散射（Dynamic Light Scattering ,DLS)，也称光子相关光谱Photon Correlation Spectroscopy (PCS) ，准弹性光散射quasi-elastic scattering，测量光强的波动随时间的变化。 DLS技术测量粒子粒径，具有准确、…

2022年10月26日 · 动态光散射（Dynamic Light Scattering），简称DLS，是一种检测胶体纳米材料整体粒径分布的有效手段。 本期，我们将主要讨论DLS检测的基本原理和三个关键问题！ 当激光照射到纳米粒子时会发生散射，由于溶液体系中的纳米粒子会进行布朗运动，小粒子运动快速，大颗粒运动缓慢，散射光的相位叠加，将引起光亮区域和黑暗区域的光强增加和减少，所以，得到的光散射强度是波动的。 DLS技术就是通过激光照射粒子，分析散射光的光强波动，然后计算 …

TEMperature测量的是干态的样品，由于干燥过程中溶剂的挥发造成的表面张力使微粒聚集，而DLS就是用来测试液体中微粒的水合半径的，能够很好地确定微粒是否聚集的一种测试手段，测试结果尺寸会大于TEM，同时聚合物微球在水中也会有一定的溶胀，尺寸也会比 ...

The particle sizes of Nano/micro-particles measured using DLS and SEM differ greatly, i.e., around 80 nm was determined by DLS, while the same sample measured according to SEM imgae showed...

2024年3月8日 · We provide a methodology based on static multiple light scattering (SMLS), dynamic light scattering (DLS), and scanning electron microscopy (SEM) measurements to characterize the NP dispersibility and stability in complex biological media.

2021年5月21日 · Dynamic light scattering (DLS) is an essential technique for nanoparticle size analysis and has been employed extensively for decades, but despite its long history and popularity, the choice of weighting and mean of the size distribution often appears to be picked ad hoc to bring the results into agreement with other methods and expectations by ...

动态光散射（DLS），亦被称为激光光散射，是一种强大的技术，能够测量小粒子，如蛋白质、纳米粒子、聚合物或脂质囊泡，在溶液或悬浮液中的尺寸。 其原理在于，当单色激光照射至被测样品时，会在特定角度产生散射光，其强度随时间变化。 由于悬浮颗粒在液体中受到分子无规则运动的撞击而进行布朗运动，因此观察到的散射光强度会随时间起伏。 利用布朗运动的斯托克斯-爱因斯坦方程，当已知悬浮液的温度和黏度时，通过分析这些散射光强度的涨落函数，我们可以计算 …

2016年8月10日 · Determination of particle size and surface charge of NPs are indispensable for proper characterization of NPs. DLS (dynamic light scattering) and ZP (zeta potential) measurements have gained popularity as simple, easy and reproducible tools to ascertain particle size and surface charge.

SEM on freeze-dried and Au-shadowed samples showed a relatively narrow distribution of virtually spherical particles with a mean diameter of 167 nm. DLS yielded a monomodal distribution with hydrodynamic diameters around 199 nm (in the absence of additional stabilizer) or 184 nm (in the presence of 1% poloxamer 188).

By combining DLS and SEM, you can gain both quantitative size distribution data (from DLS) and qualitative structural information (from SEM). This is particularly useful when working with nanoparticles, colloids, or complex particulate systems where both size and morphology are critical to understanding the material’s properties and behavior.