Cyanine3 (Cy3) is a bright, orange-fluorescent dye with excitation suited for the 532 nm laser line and is used for imaging, flow cytometry and genomics.

Because they yield brighter and more stable fluorescence, cyanines can advantageously replace conventional dyes such as fluorescein and rhodamines. Cy3 and Cy5 are the most popular, typically used combined for 2 color detection.

Cy3 is an important historical fluorophore from GE Healthcare. It has an excitation peak at 554 nm and an emission peak at 568 nm. Spectrally its similar to Alexa Fluor™ 555.

Cy3 Dye is a fluorescent dye used in 2D DIGE to covalently label proteins in samples for comparison. It allows for quantitative analysis by imaging the Cy3 signal separately and determining the labeling ratio in each spot on the gel.

Cy3 (Sulfo-Cyanine3) is an orange-fluorescent label for protein and nucleic acid (λ ex =554, λ em =568).

2024年9月5日 · Cy3® NHS Ester (GE Healthcare) and Cyanine3 monosuccinimidyl ester are chemically equivalent, reactive dyes used for fluorescence labeling biological molecules by targeting amino-groups. Cy3 is a yellow fluorescent dye belonging to the cyanine dyes family. It is sometimes also referred to as Sulfo-Cy3; the two are chemically equivalent.

5 天之前 · Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.

Cy3 dye stands as a pivotal tool in the realm of fluorescent labeling, significant not only for its vibrant fluorescence but also for its diverse applications in molecular biology and biochemistry. This article seeks to unravel the complexities surrounding Cy3 dye, from its chemical properties to its practical utility in scientific investigations.

2025年1月29日 · What Is Cy3? Cy3 is a bright, orange-fluorescent dye used for labeling proteins and nucleic acids. Why Is Cy3 Important? Cy3 is valuable for its bright fluorescence, compatibility with common imaging techniques, and wide range of applications in molecular biology. How Is Cy3 Synthesized?

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