2006年11月3日 · Lentivirus production: We have established a stable 293SF cell line (adapted to serum-free culture) expressing both the cumate repressor (CymR) and the reverse tetracycline transactivator (rtTA) for tight regulation of the packaging elements (293SF-PacLV). We co-transfected all packaging plasmids in one shot with Rev and VSV-G being doubly ...

2024年9月5日 · By utilizing both the Tet-On and cumate repressor systems in a single cell line, we generated a new system to investigate potential cooperation of PAX3::FOXO1 and HES3, with the capacity to...

2023年6月8日 · Lentiviral vectors (LVs) are important for cell therapy because of their capacity to stably modify the genome after integration. This study describes a novel and relatively simple approach to generate packaging cells and producer clones for self-inactivating (SIN) LVs pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G).

2023年7月5日 · Here, we develop Tango-Trio, a comprehensive cell-based high-throughput platform comprising cumate-inducible expression of transducers, capable of the parallelized profiling of both basal and...

Here we report a novel strategy to illuminate cell proximity using transcriptional activators. We repurposed cumate, a small molecule inducer of gene expression, by caging its key carboxylate group with a nitrile. Nitrilase-expressing activator cells released the cage, liberating cumate for consumption by reporter cells.

这项研究描述了一种新颖且相对简单的方法，用于生成用水泡性口炎病毒糖蛋白（VSV-G）假型化的自失活（SIN）LV的包装细胞和生产者克隆。开发了一种基于 cumate 和香豆霉素诱导系统组合的新型基因调控系统，以确保严格控制细胞毒性包装元件的表达。

We have developed a new inducible system for mammalian cells that can be used for applications that require tight control as well as those that require a high level of expression. We have transformed a repressor from Pseudomonas putida F1, CymR, in to an activator (cTA) by fusion to the VP16 activation domain.

2017年8月10日 · A CHO cell line containing the cumate gene switch elements (CHO Cum2) was transduced with lentiviral vectors for the generation of stable pools able to produce volumetric yields of up to 200 mg/L protein in less than two months, starting from the cDNA. Even if this method is efficient, lentiviral vectors are cumbersome to manipulate (biosafety ...

Lentiviral vectors (LVs) are important for cell therapy because of their capacity to stably modify the genome after integration. This study describes a novel and relatively simple approach to generate packaging cells and producer clones for self-inactivat-ing (SIN) LVs pseudotyped with the vesicular stomatitis virus glycoprotein (VSV-G).

2006年11月3日 · Results: We have used the regulatory mechanisms of bacterial operons (cmt and cym) to regulate gene expression in mammalian cells using three different strategies. In the repressor configuration, regulation is mediated by the binding of the repressor (CymR) to the operator site (CuO), placed downstream of a strong constitutive promoter.