目前了解最多的底物是 cullin家族 ，它是CRL（cullin-RING连接酶）复合物的支架蛋白。我们前面说过，泛素连接酶E3有3种类型，其中之一是RING类型。CRL是RING类型中最大的一个家族，需要由多种蛋白构成一个复合物。Cullin负责将其中一些组分连接起来。

Cullins are a family of hydrophobic scaffold proteins which provide support for ubiquitin ligases (E3). All eukaryotes appear to have cullins. They combine with RING proteins to form Cullin-RING ubiquitin ligases (CRLs) that are highly diverse and play a role in myriad cellular processes, most notably protein degradation by ubiquitination. [1][2]

2011年12月4日 · Cullin proteins are molecular scaffolds that have crucial roles in the post-translational modification of cellular proteins involving ubiquitin. The mammalian cullin protein family comprises eight members (CUL1 to CUL7 and PARC), which are characterized by a cullin homology domain.

2009年11月26日 · Cullins家族以及与泛素化降解肿瘤抑制基因p53相关的RING家族成为研究细胞生. 长、凋亡和肿瘤发生的重点。 本文选择RING家族中与p53相互作用的MDM2、 COP1、Pirh2进行了结构预测，并研究了这三个蛋白与泛素蛋白（Ub）的结合、 高能位点及其作用。 对于分类较明确、序列较齐全的Cullins家族，本文从动物中. 选取了172条序列，构建了Cullins家族及各亚家族的系统发育树。 除此之外，本. 文还将现存的所有cullin家族序列进行收集和比对，确定了各家 …

其中Cullin-RING E3 连接酶(Cullin-RING E3ligase, CRL)是最大的E3泛素连接酶超家族，负责细胞内约20%的蛋白质通过UPS降解。CRL是一种多亚基复合体，通常由骨架蛋白Cullin、RING-box蛋白、衔接蛋白和底物识别蛋白四个部分组成。

2011年12月4日 · Cullin proteins are molecular scaffolds that have crucial roles in the post-translational modification of cellular proteins involving ubiquitin. The mammalian cullin protein family comprises eight members (CUL1 to CUL7 and PARC), which are characterized by a cullin homology domain.

Cullin蛋白通常通过接头蛋白连接底物靶向单位和CRL中的环指成分，CRLS以多种底物为靶标，影响一系列生物学过程，包括细胞生长、发育、信号转导、转录调控、基因组完整性和肿瘤抑制 [3] 。

2005年1月1日 · In this review, we focus on the composition, regulation and function of cullin–RING ligases, and describe how these enzymes can be characterized by a set of general principles.

Cullins (CULs) are a core component of cullin-RING E3 ubiquitin ligases (CRLs), which regulate the degradation, function, and subcellular trafficking of proteins. CULs are post-translationally regulated through neddylation, a process that conjugates ...

Cullin proteins are molecular scaffolds that assemble multi-subunit cullin-RING E3 ubiquitin ligase (CRL) complexes. The mammalian cullin protein family comprises eight members (CUL1 to CUL7 and PARC). In CRL, a cullin protein tethers both a substrate-recognition subunit, often through an adaptor protein, and the RING finger component.