Cre-loxP recombination allows scientists to excise, insert, or invert specific DNA segments with unprecedented accuracy. This works through two key components: a Cre recombinase and loxP sequence recognition sites.

This review provides a brief overview of Cre-loxP system and a few commonly used promoters for expression of tissue-specific Cre recombinase. Also, we finally introduce some available links to the Web sites that provides detailed information about Cre mouse lines including their characterization.

2017年3月2日 · The cre-loxP-mediated recombination system (the "cre-loxP system") is an integral experimental tool for mammalian genetics and cell biology. Use of the system has greatly expanded our ability to precisely interrogate gene function in the mouse, providing both spatial and temporal control of gene exp …

Here is the most efficient breeding scheme for generating Cre/ lox tissue-specific or inducible knockouts. To generate mice that are heterozygous for a loxP -flanked allele and hemizygous/heterozygous for the cre transgene, mate a homozygous loxP -flanked mouse of interest to a cre transgenic mouse strain (see Figure 1 below).

6 天之前 · The Cre-loxP system is a potent tool that is used in an array of life science fields to address molecular and cellular mechanisms in heredity, development, physiology, disease, and more.Cre is a ...

Cre- loxP recombination allows scientists to excise, insert, or invert specific DNA segments with unprecedented accuracy. 1,2 This works through two key components: a Cre recombinase and loxP sequence recognition sites.

The Cre-lox system is a technology that can be used to induce site-specific recombination events. The system consists of two components derived from the P1 bacteriophage: the Cre recombinase and a loxP recognition site.

Cre recombinase is a phage-derived enzyme that has found utility for precise manipulation of DNA sequences. Cre recognizes and recombines pairs of loxP sequences characterized by an inverted repeat and asymmetric spacer. Cre cleaves and religates its DNA targets such that error-prone repair pathways are not required to generate intact DNA products.

2020年10月20日 · Cyclization recombinase (Cre) is one of the tyrosine site-specific recombinases, which is known to catalyse the site specific recombination event between two DNA recognition sites (LoxP sites). Cre recombinase consists of 343 amino acids, which can specifically recognize Lox sites.

Cre recombinase (Cre) is a 343-amino-acid protein comprised of 4 subunits that recognizes pairs of specific 34 bp DNA sequences called loxP sites. It initially creates a DNA loop and then either excises or inverts the looped segment depending on the orientation of the loxP sites [ 1 – 3 ].