Chemically induced dimerization (CID) is a biological mechanism in which two proteins bind only in the presence of a certain small molecule, enzyme or other dimerizing agent. [1] Genetically engineered CID systems are used in biological research to control protein localization, to manipulate signalling pathways and to induce protein activation.

2023年8月28日 · A new chemically induced dimerization (CID) pair exhibits fluorescence upon dimerization for the first time. Moreover, the CID pair is small and offers easily reversible dimerization that can...

2018年3月9日 · Chemically induced proximity allows for precise temporal control of transcription, signaling cascades, chromatin regulation, protein folding, localization, and degradation, as well as a host of other biologic processes.

2020年8月3日 · Chemically inducible dimerization (CID) uses a small molecule to induce binding of two different proteins. CID tools such as the FK506-binding protein–FKBP–rapamycin-binding– (FKBP–FRB)–rapamycin...

We describe the development and application of a peptide template-induced dimerization strategy to facilitate in situ production of bioactive ligand for PTHR1 from weakly active and inactive building blocks.

2023年8月28日 · Here we present CATCHFIRE (chemically assisted tethering of chimera by fluorogenic-induced recognition), a chemically induced proximity technology with intrinsic fluorescence imaging and...

2015年10月1日 · Small-molecule perturbation of biological systems is able to tackle biological problems that are not accessible by classical genetic interference methods. Chemically induced dimerization (CID) has been used as a valuable tool to study various biological processes.

Currently, only a limited number of chemically induced dimerization (CID) systems exist and engineering new ones with desired sensitivity and selectivity for specific small-molecule ligands remains a challenge in the field of protein engineering.

Chemically induced dimerization (CID) systems, in which two proteins dimerize only in the presence of a small molecule ligand, offer versatile tools for small molecule sensing and actuation. Howeve...

2025年2月13日 · Chemically induced dimerization is an important tool for the external regulation of biological processes in mammalian cells. Here, we describe a chemogenetic platform based on the conditional reassembly of antibody variable fragments, which we call Fv-CID switches. Those molecular switches allow for chemical regulation of gene expression as ...