2021年5月29日 · Feeding a CDAHFD diet for only 1-week induced lipid droplet deposition in the liver with increasing activity of liver-derived enzymes in the plasma. On the other hand, it did not induce fibrosis or cirrhosis.

2024年3月18日 · A choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) has recently been used to induce the NASH model, but the advantages are not established. NASH models were induced using the well-known traditional methionine- and choline-deficient (MCD) diet for 5 weeks and the recently used CDAHFD for 3 weeks.

In the present study, we developed a choline-deficient, L-amino-acid-defined, high-fat-diet (CDAHFD)-induced human NASH model using human liver chimeric mice. We demonstrated human hepatocyte injury by an elevation of plasma human alanine aminotransferase 1 …

Here, we analyzed the effects of dietary methionine content on NASH induced in mice fed on a choline‐deficient, methionine‐lowered, l ‐amino acid‐defined high‐fat diet (CDAHFD). CDAHFD with insufficient methionine induced insulin sensitivity and …

2021年3月17日 · Mice were fed a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) to induce NASH with advanced fibrosis. Mice fed normal chow and CDAHFD underwent MRI after 2, 6, 10 and 14 weeks to...

最近已使用缺乏胆碱、 L-氨基酸限定的高脂肪饮食（CDAHFD）来诱导 NASH 模型，但其优点尚未确立。 NASH模型的诱导使用著名的传统蛋氨酸和胆碱缺乏（MCD）饮食5周，以及最近使用的CDAHFD 3周。 分析肝脏表型以评估与 NASH 相关的标志物的差异。 脂质组学和代谢分析用于研究饮食方案对肝脏脂质组的影响。 CDAHFD 比 MCD 诱导更强的 NASH 反应，包括脂质沉积 …

2024年11月29日 · CDAHFD-fed mice exhibited local cholesterol accumulation and a pro-inflammatory macrophage phenotype in the liver. Cholesterol overload in vitro promoted M1 polarization and liver inflammation, reversible by simvastatin.

2024年11月10日 · Organoids were developed using mouse liver tissues subjected to a choline-deficient L-amino acid-defined high-fat diet (CDAHFD) to mimic MASH-related HCC. The study evaluated the effect of...

2022年12月20日 · In this line, preclinical mouse models are crucial tools to investigate mechanisms as well as novel treatment modalities during the pathogenesis of NASH and subsequent HCC in preparation for...

These results demonstrated that 1 week of feeding CDAHFD to mice induces steatohepatitis with mitochondrial dysfunction and severe oxidative stress, without fibrosis, which can partially mimic the early stage of NASH in humans. 1. Introduction.

60%高脂肪0.1%蛋氨酸的胆碱缺乏 (CDAHFD)模型饲料用于非酒精性脂肪肝模型 (NASH)的造模，不仅NASH表现与人类相似，而且具有体重不下降、发展速度快的优点，在肝纤维化研究及药物作用研究中具有特别优势。 这里介绍该模型饲料（Matsumoto diet）的情况和造模方法。

已开发出胆碱缺乏氨基酸定义的高脂饮食（CDAHFD）诱导的NASH小鼠模型。 但是，其与人类NASH的相关性，包括血清脂质谱，尚未得到明确定义。 在这项研究中，我们已经发现，饲喂CDAHFD的小鼠除体重（BW）降低外，还显着降低了血清总胆固醇和甘油三酸酯（TG）的水平。 此外，喂食CDAHFD的小鼠肝微粒体甘油三酸酯转移蛋白（MTP）的表达明显下调。 因此， …

In this study, we have revealed that mice fed CDAHFD showed significantly lowerd serum total cholesterol and triglyceride (TG) levels, in addition to reduced body weight (BW). Furthermore, hepatic microsomal triglyceride transfer protein (MTP) expression was significantly downregulated in CDAHFD-fed mice.

We aimed to establish a mouse model of NASH with fibrosis by optimizing the methionine content in the HFD. Male mice were fed a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) consisting of 60 kcal% fat and 0.1% methionine by weight. After 1–14 weeks of being fed CDAHFD, the mice were killed.

2024年1月29日 · Here, we determined whether TRF affects NASH pathology induced by a choline-deficient high-fat diet (CDAHFD), which does not involve obesity. TRF ameliorated the increase in epididymal white adipose tissue and plasma alanine transaminase and aspartate transaminase levels after 8 weeks of a CDAHFD.

We have previously reported a choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) as a dietary NASH model with rapidly progressive liver fibrosis in mice.

In this review, we summarize the newly described patho-mechanisms of NAFLD and review the preclinical mouse models of NAFLD (based on the method of induction) and appraises the use of these models in anti-NAFLD drug discovery.

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2020年12月16日 · To profile the regulatory network of PPAR α/δ agonist in NASH, the choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) induced NASH model was used to test the pharmacodynamics and transcriptome regulation of GFT505 in this study.