Cas9 (CRISPR associated protein 9, formerly called Cas5, Csn1, or Csx12) is a 160 kilodalton protein which plays a vital role in the immunological defense of certain bacteria against DNA …

2022年10月1日 · In addition, it efficiently transported Cas9 ribonucleoprotein (RNP) into cells for CRISPR/Cas9 gene editing and achieved liver repair against acetaminophen (APAP)-induced …

2018年5月8日 · The cell-penetrating α-helical polypeptide enhanced cellular uptake and promoted escape of pCas9 and/or sgRNA from the endosome and transport into the nucleus. The …

Cas9 is unique in that it is composed of a single polypeptide that utilizes both a crRNA and a trans-activating crRNA (tracrRNA) or a single guide RNA to create double-stranded breaks in …

Cas9 has been harnessed as a powerful tool for genome editing and gene regulation in many eukaryotic organisms. We report 2.6 and 2.2 angstrom resolution crystal structures of two …

2024年12月25日 · A guanidyl-decorated α-helical polypeptide (LPP) was employed not only to encapsulate A-protein through electrostatic attraction and hydrogen bonding, forming stable …

2023年2月10日 · Cas9 is a bacterial RNA-guided endonuclease that cleaves target DNA and modifies a cell's genome. Despite the advantages and promising results of the use of CRISPR …

2018年5月5日 · We report an efficient CRISPR/Cas9 delivery system comprising PEGylated nanoparticles based on the α-helical polypeptide PPABLG. Assisted by the high membrane …

2019年3月20日 · Successful and efficient delivery of Cas9 protein and gRNA into cells is critical for genome editing and its therapeutic application. In this study, we developed an improved …

2021年6月1日 · We have developed a one-step strategy using engineered lentiviral particles to introduce Cas9 RNPs and a CAR transgene into primary human T cells without …