2020年7月17日 · CasΦ (Cas12j) is a family of Cas proteins encoded in the Biggiephage clade (4). CasΦ contains a C-terminal RuvC domain with remote homology to that of the TnpB nuclease superfamily from which type V CRISPR-Cas proteins are thought to have evolved (4, 5) (fig. S1).

2023年2月10日 · We experimentally proved that Cas12j-8 enabled allele-specific disruption of genes with a single-nucleotide polymorphism (SNP). Cas12j-8 recognizes a simple TTN PAM that provides for high target site density.

2021年7月22日 · CRISPR-Cas12j is a recently identified family of miniaturized RNA-guided endonucleases from phages. These ribonucleoproteins provide a compact scaffold gathering all key activities of a...

2023年11月21日 · We aimed to identify Cas12j family members with high inherent gene editing efficiency from an environmental metagenome, to broaden the range of Cas12j-based CRISPR tools for biotechnological...

2023年2月10日 · Cas12j-8 is a highly specific nuclease sensitive to single-nucleotide mismatches in the protospacer adjacent motif (PAM)-proximal region. We experimentally proved that Cas12j-8 enabled allele-specific disruption of genes with a single-nucleotide polymorphism (SNP).

Here, the trans‐cleavage behavior of Cas12j1, 2, and 3 variants and evaluate their suitability for nucleic acid detection is unveiled. The target preferences and mismatch specificities of the Cas12j variants are precisely investigated and the optimal Cas12j reaction conditions are determined.

Here, the trans-cleavage behavior of Cas12j1, 2, and 3 variants and evaluate their suitability for nucleic acid detection is unveiled. The target preferences and mismatch specificities of the Cas12j variants are precisely investigated and the optimal Cas12j reaction conditions are determined.

2022年5月19日 · Cas12c2 and Cas12j (Pausch et al., 2020) process their pre-crRNAs at their RuvC domains. Our comparisons of the binary complex structures of Cas12c2 and Cas12j (Pausch et al., 2021) revealed some structural differences between them (Figures S6A and S6B).

2021年9月2日 · In the present study, we redesigned the natural guide RNA of Un1Cas12f1 at five sites: the 5′ terminus of the trans -activating CRISPR RNA (tracrRNA), the tracrRNA–crRNA complementary region, a...

CRISPR-Cas12j is a compact and highly specific nuclease, which would be particularly suitable for therapeutic applications. The RNA-guided CRISPR-Cas system is a prokaryotic adaptive immune system that has been used for mammalian genome editing (1, 2).