C2C12 is a myoblast cell line that is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.

The C2C12 cell model is a well-established in vitro system used to study muscle cell biology, including myogenesis (muscle formation), gene expression, and muscle metabolism. C2C12 cells can differentiate into myotubes under low serum conditions.

2022年1月17日 · Intracellular metabolite profiles indicated that C2C12 myotubes cultured in serum and B27 had predominant glycolytic and oxidative metabolism, respectively, indicating fast and slow types of...

2011年5月17日 · The cell line C2C12 is an immortal line of mouse skeletal myoblasts originally derived from satellite cells from the thigh muscle of a two month old female C3H mouse donor 70h after a crush injury (Yaffe and Saxel, 1977; karyotyping available in Casas-Delucchi, 2011).

The C2C12 cell line, derived from mouse skeletal muscle myoblasts established by Yaffe and Saxel [22], is a common precursor cell model for skeletal muscle research, particularly in the study of muscle atrophy [23,24]. These cells exhibit strong proliferation and differentiation capabilities and can be induced to differentiate under low ...

2024年12月25日 · C2C12 (mouse myoblasts) is a subclone of a mouse myoblast cell line established by D. Yaffe and O. Saxel. C2C12 cells differentiate rapidly, readily forming contractile myotubes and generating characteristic muscle proteins.

In contrast, when C2C12 cells are stimulated with ligands or type I receptors, Smad5 is mainly observed in the nucleus [7]. These findings indicate that nuclear translocation induced by receptor activation is required to elicit the biological activity of R-Smads.

Mouse C2C12 muscle cells are the most commonly used cellular model to mimic skeletal muscle in vitro. Various studies reporting the effects of myostatin on C2C12 proliferation and differentiation exist [17, 18, 19, 20, 21].

We examined the myofibril biochemical, structural, and biophysical properties of C2C12, a mouse skeletal muscle cell line (American Type Culture Collection), to assess whether force development and the sensitivity of the myofilaments to calcium could be measured in C2C12 myotubes and whether a cardiac contractile protein, troponin T, is ...

2017年3月16日 · To verify the involvement of follistatin in myogenic differentiation and contractile force generation, we created genetically-engineered C2C12 cells capable of Dox-inducible follistatin...