2024年5月22日 · This colorimetric chemical test used to detect the peptide bond using the Biuret reagent is called the Biuret test. It is also called Piotrowski’s reaction after the name of the Polish physiologist Gustaw Piotrowski who observed this phenomenon in 1857 and used it to detect proteins in samples.

In chemistry, the biuret test (IPA: / ˌ b aɪ j ə ˈ r ɛ t /, / ˈ b aɪ j ə ˌ r ɛ t / [1]), also known as Piotrowski's test, is a chemical test used for detecting the presence of at least two peptide bonds in a molecule.

The biuret method is a colorimetric technique specific for proteins and peptides. Copper salts in alkaline solution form a purple complex with substances containing two or more peptide bonds.

Prepare a standard curve of absorbance versus micrograms protein (or vice versa), and determine amounts from the curve. Determine concentrations of original samples from the amount protein, volume/sample, and dilution factor, if any. The color is stable, but all readings should be taken within 10 min. of each other.

The biuret test is a colorimetric test that helps detect specific proteins or peptide bonds in given analytes. It is followed by spectrophotometry for quantification. The test requires the use of a biuret reagent.

2024年1月1日 · Aim: To estimate the concentration of protein using Biuret method. Principle: The is a colorimetric estimation technique that is specific for proteins and peptides used for estimation of protein. The coppers salt reacts with the two more peptide bonds under alkaline condition result in purple complex.

2025年2月22日 · The biuret test is a widely used method to detect and quantify proteins in a solution. When the biuret reagent is added to a sample containing proteins, it reacts with the nitrogen atoms in the peptide bonds, forming a purple-colored complex. This reaction is specific to proteins and is the basis for their detection.

2023年2月19日 · The Biuret Test for Protein is a simple and reliable method to detect the presence of proteins in a sample. It is based on the principle that copper ions in the Biuret reagent react with peptide bonds in proteins to form a violet-coloured complex.

2021年4月9日 · The biuret method is a colorimetric technique specific for proteins and peptides. Copper salts in alkaline solution form a purple complex with substances containing two or more peptide bonds. In other words, Biuret test is based on the reaction of the cupric ions Cu 2+ with peptide bonds in an alkaline solution.

2018年4月20日 · Biuret is a compound formed by heating urea to 180° C. When biuret is treated with dilute copper sulfate in alkaline condition, a purple colored compound is formed. This is the basis of biuret test widely used for identification of proteins and amino acids. This test is given by compounds containing two or more peptide bond (CO-NH group).