avGFP is a basic (constitutively fluorescent) long stokes shift fluorescent protein published in 1992, derived from Aequorea victoria. It has low acid sensitivity.

2021年4月10日 · 大家在观察荧光蛋白表达之前，一定要看自己质粒图谱用的到底是哪种GFP，如果是GFP、wtGFP、avGFP必须用紫外光源去激发（365nm波段，LUYOR-3280UV），如果的eGFP、mEGFP那要蓝光波段(450nm LUYOR-3280RB)去激发。

摘要 1962年从水母Aequorea victoria中发现并提取出的绿色荧光蛋白（green fluorescent protein，GFP）以及1999年从珊瑚Discosoma genus中发现的红色荧光蛋白（drFP583）现已成为在生物研究中的各个领域最为广泛的蛋白质之一。 由于其具有对生物体无毒无害、受特定波长光激发时可高效发射特征荧光、分子量小、易于构建载体以及在多种生物体中均可表达的特性，在众多领域中展现出了广阔的前景。 现已有多种荧光蛋白被应用于实际研究中。 关键词 荧光蛋白， …

Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expr...

2024年7月30日 · This study aims to utilize Transformer models and large language models (such as GPT and Claude) to predict the brightness of Aequorea victoria green fluorescent protein (avGFP) and design mutants...

2018年12月1日 · The green fluorescent protein from Aequorea victoria (avGFP) and its homologous fluorescent proteins (FPs) are now the most commonly used probes to visualize the spatiotemporal dynamics of cells [25, 26]. These fluorophores are genetically encoded, and consequently, no loading procedure is necessary, which ensures noninvasive imaging.

2016年5月11日 · Here we visualize an extensive region of the local fitness landscape of the green fluorescent protein from Aequorea victoria (avGFP) by measuring the native function (fluorescence) of tens of...

2019年10月13日 · 通过定点突变和随机突变得到了不同突变类型的avGFP样蛋白，珊瑚类荧光蛋白的发现使人们发展出更多性质各异的荧光蛋白,发射谱覆盖420~655nm，应用范围不断扩大。

avGFP (wt GFP) excitation: 395 nm and emission: 509 nm. avGFP is a 26.9 kDa fluorescent protein derived from Aequorea victoria. Learn more about avGFP.

2017年2月1日 · The initial demonstration in 1994 that the Aequorea victoria jellyfish (class Hydrozoa) green fluorescent protein (avGFP; Figure 1 A) could function as a genetically encodable fluorescent tag [1] was followed by rapid-fire protein-engineering efforts to fine tune its biochemical and fluorescent properties and expand the color palette to ...